Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3275005..3275625 Replicon chromosome
Accession NZ_CP009492
Organism Yersinia pestis strain PBM19

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag CH59_RS16060 Protein ID WP_002208622.1
Coordinates 3275005..3275208 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q66DR4
Locus tag CH59_RS16065 Protein ID WP_002218472.1
Coordinates 3275257..3275625 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CH59_RS24235 3270279..3270365 + 87 Protein_2793 hypothetical protein -
CH59_RS16040 3270405..3271694 + 1290 WP_002228344.1 ammonium transporter AmtB -
CH59_RS16045 3271954..3272814 - 861 WP_002208625.1 acyl-CoA thioesterase II -
CH59_RS16050 3273071..3273592 + 522 WP_002208624.1 lipoprotein -
CH59_RS16055 3273728..3274117 - 390 WP_002208623.1 MGMT family protein -
CH59_RS16060 3275005..3275208 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
CH59_RS16065 3275257..3275625 - 369 WP_002218472.1 Hha toxicity modulator TomB Antitoxin
CH59_RS16070 3275784..3276137 - 354 WP_002208619.1 hypothetical protein -
CH59_RS16075 3277011..3277154 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
CH59_RS16080 3277170..3277430 - 261 WP_002208617.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T49166 WP_002208622.1 NZ_CP009492:c3275208-3275005 [Yersinia pestis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T49166 NZ_CP009492:c3275208-3275005 [Yersinia pestis]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACGATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14215.00 Da        Isoelectric Point: 4.6121

>AT49166 WP_002218472.1 NZ_CP009492:c3275625-3275257 [Yersinia pestis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Download         Length: 369 bp

>AT49166 NZ_CP009492:c3275625-3275257 [Yersinia pestis]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB Q66DR4

References