Detailed information of TA system TA049163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 42368..42902
• Replicon: plasmid pCD1
• Accession: NZ_CP009491
• Organism: Yersinia pestis strain PBM19

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: CH59_RS00900
• Protein ID: WP_002213360.1
• Coordinates: 42368..42667 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: CH59_RS00905
• Protein ID: WP_002224342.1
• Coordinates: 42660..42902 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH59_RS00865	38063..39082	+	1020	Protein_49	IS21-like element IS100 family transposase	-
CH59_RS00870	39082..39858	+	777	Protein_50	IS21-like element IS100kyp family helper ATPase IstB	-
CH59_RS25275	39925..40173	-	249	Protein_51	IS21 family transposase	-
CH59_RS00885	40276..40920	+	645	Protein_52	IS3 family transposase	-
CH59_RS00890	40983..41375	-	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
CH59_RS00895	41569..42228	+	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
CH59_RS00900	42368..42667	-	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH59_RS00905	42660..42902	-	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CH59_RS24745	43068..43360	-	293	Protein_57	IS6 family transposase	-
CH59_RS26055	43447..43620	+	174	WP_002213357.1	hypothetical protein	-
CH59_RS25855	43769..44005	-	237	WP_154020274.1	hypothetical protein	-
CH59_RS00915	44165..45130	-	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
CH59_RS00920	45127..46293	-	1167	WP_002220902.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yscR / yscS / yscT / yscU / virG/yscW / yscB / yscC / yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / yopH / yopJ/yopP / yopO/ypkA / yadA / yadA / sycH / sycE/yerA / yopE / yopK/yopQ / yopT / sycT / yopM / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN / yscN / yscN / yscO / yscP / yscQ	1..70288	70288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T49163 WP_002213360.1 NZ_CP009491:c42667-42368 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T49163 NZ_CP009491:c42667-42368 [Yersinia pestis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT49163 WP_002224342.1 NZ_CP009491:c42902-42660 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT49163 NZ_CP009491:c42902-42660 [Yersinia pestis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6