Detailed information of TA system TA049159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2629211..2629866
• Replicon: chromosome
• Accession: NZ_CP009486
• Organism: Burkholderia ubonensis MSMB22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BW23_RS11305
• Protein ID: WP_045565215.1
• Coordinates: 2629211..2629630 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A124REA9
• Locus tag: BW23_RS11310
• Protein ID: WP_042588282.1
• Coordinates: 2629627..2629866 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW23_RS11280	2625161..2625544	+	384	Protein_2324	permease	-
BW23_RS11285	2625837..2626862	-	1026	WP_042584526.1	IS110 family transposase	-
BW23_RS11290	2627004..2627138	-	135	WP_029226154.1	entericidin A/B family lipoprotein	-
BW23_RS11295	2627370..2628665	+	1296	WP_010089460.1	aspartate carbamoyltransferase	-
BW23_RS11300	2628666..2629127	+	462	WP_198364311.1	hypothetical protein	-
BW23_RS11305	2629211..2629630	-	420	WP_045565215.1	type II toxin-antitoxin system VapC family toxin	Toxin
BW23_RS11310	2629627..2629866	-	240	WP_042588282.1	Arc family DNA-binding protein	Antitoxin
BW23_RS11315	2630178..2632415	+	2238	WP_045565216.1	TonB-dependent siderophore receptor	-
BW23_RS11320	2632419..2633174	+	756	WP_045565217.1	sel1 repeat family protein	-
BW23_RS11325	2633171..2633854	+	684	WP_045565218.1	Fe2+-dependent dioxygenase	-
BW23_RS11330	2633979..2634218	+	240	WP_011659184.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BW23_RS11335	2634215..2634610	+	396	WP_042588895.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2625254..2636831	11577
	-	flank	IS/Tn	-	-	2625837..2626862	1025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14978.28 Da
• Isoelectric Point: 5.8883

>T49159 WP_045565215.1 NZ_CP009486:c2629630-2629211 [Burkholderia ubonensis MSMB22]
MILVDTNVLSEPLRPAPNASVLEWLDAQNVETLFLAAISLAELRFGVAAMPEGKRRDWLHRSIEQRVMPAFRGRILPFDA
AASEAYAKLRARARAAGSVIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAV

Nucleotide

• Length: 420 bp

>T49159 NZ_CP009486:c2629630-2629211 [Burkholderia ubonensis MSMB22]
ATGATTCTTGTCGATACCAACGTCTTGTCCGAGCCGCTGCGGCCTGCGCCGAATGCGTCAGTGCTGGAGTGGCTCGATGC
GCAGAATGTCGAAACGTTGTTTCTCGCGGCGATCAGCCTGGCCGAGTTGAGATTCGGCGTCGCCGCCATGCCGGAAGGCA
AGCGGCGAGACTGGCTGCATCGAAGCATCGAACAACGCGTGATGCCGGCGTTTCGCGGCCGGATCCTGCCGTTCGACGCG
GCTGCGAGCGAGGCCTATGCCAAGCTTCGCGCCCGCGCGCGGGCGGCCGGCAGCGTGATCGCGCCGGCCGACGGCTATAT
CGCCGCGACTGCCGAAGCCAATGGATTGATCGTGGCGACGCGCGACGTCGCGCCGTTTGAAGCGGCCGGCCTGCGGGTGA
TCGATCCGTGGGCGGTGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8840.10 Da
• Isoelectric Point: 5.7135

>AT49159 WP_042588282.1 NZ_CP009486:c2629866-2629627 [Burkholderia ubonensis MSMB22]
MPVITVRNLPDEVHRALRVRAAQHGRSTEAEVRDILEKAVQPEGRVKLGTLLAEIGREIGGVDLDIQRDKTPTEPMNFE

Nucleotide

• Length: 240 bp

>AT49159 NZ_CP009486:c2629866-2629627 [Burkholderia ubonensis MSMB22]
ATGCCCGTGATAACCGTTCGGAATTTGCCTGACGAAGTCCATCGCGCCCTTCGGGTTCGCGCGGCGCAGCATGGACGCAG
CACCGAAGCGGAGGTGCGTGACATCCTCGAGAAAGCGGTTCAGCCGGAAGGGCGCGTCAAATTGGGAACGTTGCTGGCGG
AAATCGGGCGGGAAATCGGGGGTGTCGATCTCGACATTCAGCGCGACAAAACCCCAACCGAGCCGATGAACTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A124REA9