49151

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 737199..737736 Replicon chromosome
Accession NZ_CP009484
Organism Burkholderia pseudomallei MSHR491

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BG17_RS03120 Protein ID WP_004549731.1
Coordinates 737199..737468 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BG17_RS03125 Protein ID WP_004525420.1
Coordinates 737452..737736 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG17_RS41505 733494..733922 - 429 Protein_619 DDE-type integrase/transposase/recombinase -
BG17_RS41510 733920..734693 - 774 Protein_620 prohibitin family protein -
BG17_RS03100 734690..734839 - 150 WP_004537646.1 bacteriophage protein Gp44 -
BG17_RS03105 735090..735680 + 591 WP_038800004.1 hypothetical protein -
BG17_RS40940 735855..736073 - 219 WP_004552392.1 hypothetical protein -
BG17_RS32955 736371..736646 - 276 WP_038749212.1 hypothetical protein -
BG17_RS03120 737199..737468 + 270 WP_004549731.1 BrnT family toxin Toxin
BG17_RS03125 737452..737736 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BG17_RS32970 738383..738790 + 408 Protein_627 DDE-type integrase/transposase/recombinase -
BG17_RS03135 738851..739861 - 1011 Protein_628 IS3 family transposase -
BG17_RS03145 739928..740947 + 1020 WP_038800003.1 IS110 family transposase -
BG17_RS03150 741174..741290 - 117 Protein_630 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T49151 WP_004549731.1 NZ_CP009484:737199-737468 [Burkholderia pseudomallei MSHR491]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T49151 NZ_CP009484:737199-737468 [Burkholderia pseudomallei MSHR491]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT49151 WP_004525420.1 NZ_CP009484:737452-737736 [Burkholderia pseudomallei MSHR491]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT49151 NZ_CP009484:737452-737736 [Burkholderia pseudomallei MSHR491]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References