Detailed information of TA system TA049150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 684367..684904
• Replicon: chromosome
• Accession: NZ_CP009484
• Organism: Burkholderia pseudomallei MSHR491

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BG17_RS02855
• Protein ID: WP_009923450.1
• Coordinates: 684367..684636 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BG17_RS02860
• Protein ID: WP_004525420.1
• Coordinates: 684620..684904 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG17_RS02820	679387..680208	+	822	WP_004549250.1	hypothetical protein	-
BG17_RS02825	680205..680465	+	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
BG17_RS02830	680619..681611	+	993	WP_038800019.1	hypothetical protein	-
BG17_RS02835	681608..682144	+	537	WP_038800020.1	DUF1064 domain-containing protein	-
BG17_RS02840	682169..682498	+	330	WP_024429362.1	winged helix-turn-helix domain-containing protein	-
BG17_RS32880	682495..682866	+	372	WP_076901423.1	DUF2591 family protein	-
BG17_RS32885	682863..683306	+	444	WP_076901422.1	DUF1364 family protein	-
BG17_RS02845	683617..683877	+	261	WP_038725177.1	hypothetical protein	-
BG17_RS02850	683874..684317	+	444	WP_038800021.1	hypothetical protein	-
BG17_RS02855	684367..684636	+	270	WP_009923450.1	BrnT family toxin	Toxin
BG17_RS02860	684620..684904	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BG17_RS02870	685158..685544	-	387	WP_004552941.1	helix-turn-helix domain-containing protein	-
BG17_RS32900	685541..685798	-	258	WP_004549735.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG17_RS02875	685857..686213	+	357	WP_004548856.1	HNH endonuclease	-
BG17_RS02880	686361..686846	+	486	WP_038725171.1	phage terminase small subunit P27 family	-
BG17_RS02885	686856..688568	+	1713	WP_038725169.1	terminase large subunit	-
BG17_RS02890	688565..688750	+	186	WP_038725166.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	661191..733485	72294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T49150 WP_009923450.1 NZ_CP009484:684367-684636 [Burkholderia pseudomallei MSHR491]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T49150 NZ_CP009484:684367-684636 [Burkholderia pseudomallei MSHR491]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT49150 WP_004525420.1 NZ_CP009484:684620-684904 [Burkholderia pseudomallei MSHR491]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT49150 NZ_CP009484:684620-684904 [Burkholderia pseudomallei MSHR491]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8