Detailed information of TA system TA049148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 51205..51694
• Replicon: chromosome
• Accession: NZ_CP009478
• Organism: Burkholderia pseudomallei MSHR2543

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BG16_RS13335
• Protein ID: WP_038734759.1
• Coordinates: 51205..51426 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q2T6G1
• Locus tag: BG16_RS13340
• Protein ID: WP_011401115.1
• Coordinates: 51410..51694 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG16_RS13295	47018..47278	+	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
BG16_RS13300	47432..48424	+	993	WP_038796645.1	hypothetical protein	-
BG16_RS13305	48421..48957	+	537	WP_038796646.1	DUF1064 domain-containing protein	-
BG16_RS13310	48967..49311	+	345	WP_143282741.1	winged helix-turn-helix domain-containing protein	-
BG16_RS13315	49308..49655	+	348	WP_038738298.1	DUF2591 family protein	-
BG16_RS13320	49670..50095	+	426	WP_004526654.1	DUF1364 family protein	-
BG16_RS13325	50406..50666	+	261	WP_038725177.1	hypothetical protein	-
BG16_RS13330	50663..51106	+	444	WP_038796648.1	hypothetical protein	-
BG16_RS13335	51205..51426	+	222	WP_038734759.1	BrnT family toxin	Toxin
BG16_RS13340	51410..51694	+	285	WP_011401115.1	BrnA antitoxin family protein	Antitoxin
BG16_RS41475	51691..51855	+	165	WP_004549060.1	hypothetical protein	-
BG16_RS13350	51952..52338	-	387	WP_004552941.1	helix-turn-helix domain-containing protein	-
BG16_RS36405	52335..52592	-	258	WP_004549735.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG16_RS13355	52651..53007	+	357	WP_004548856.1	HNH endonuclease	-
BG16_RS13360	53155..53640	+	486	WP_076861667.1	phage terminase small subunit P27 family	-
BG16_RS13365	53650..55362	+	1713	WP_038796649.1	terminase large subunit	-
BG16_RS13370	55359..55544	+	186	WP_038725166.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	22261..85010	62749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (14-65)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8606.71 Da
• Isoelectric Point: 8.4920

>T49148 WP_038734759.1 NZ_CP009478:51205-51426 [Burkholderia pseudomallei MSHR2543]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREAKSYVEQA

Nucleotide

• Length: 222 bp

>T49148 NZ_CP009478:51205-51426 [Burkholderia pseudomallei MSHR2543]
ATGGAGTGTCGTTGGCGCTTGCAGCGCAACTCGACTGGTCAGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAG
TGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGC
ACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGCCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10669.09 Da
• Isoelectric Point: 5.8930

>AT49148 WP_011401115.1 NZ_CP009478:51410-51694 [Burkholderia pseudomallei MSHR2543]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT49148 NZ_CP009478:51410-51694 [Burkholderia pseudomallei MSHR2543]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAAGGAGCACCAGCCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q2T6G1