Detailed information of TA system TA049145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 736114..736555
• Replicon: chromosome
• Accession: NZ_CP009477
• Organism: Burkholderia pseudomallei MSHR2543

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BG16_RS03000
• Protein ID: WP_017881988.1
• Coordinates: 736114..736287 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BG16_RS03005
• Protein ID: WP_004525420.1
• Coordinates: 736271..736555 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG16_RS02965	732963..733511	-	549	Protein_592	prohibitin family protein	-
BG16_RS02970	733508..733657	-	150	WP_004537646.1	bacteriophage protein Gp44	-
BG16_RS02975	733908..734498	+	591	WP_004552393.1	hypothetical protein	-
BG16_RS02990	735048..735179	-	132	WP_009923452.1	bacteriophage protein Gp48	-
BG16_RS02995	735189..735464	-	276	WP_004525419.1	hypothetical protein	-
BG16_RS03000	736114..736287	+	174	WP_017881988.1	BrnT family toxin	Toxin
BG16_RS03005	736271..736555	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BG16_RS03015	736941..737324	+	384	Protein_599	DDE-type integrase/transposase/recombinase	-
BG16_RS33460	737287..737715	+	429	Protein_600	IS110 family transposase	-
BG16_RS03020	737942..738058	-	117	Protein_601	transposase	-
BG16_RS03025	738305..739765	+	1461	WP_004538070.1	metallophosphoesterase	-
BG16_RS03030	739924..741186	+	1263	WP_004530738.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	725547..743408	17861
	-	inside	IScluster/Tn	-	-	731771..743408	11637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6663.54 Da
• Isoelectric Point: 7.9950

>T49145 WP_017881988.1 NZ_CP009477:736114-736287 [Burkholderia pseudomallei MSHR2543]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREAKSYVEQA

Nucleotide

• Length: 174 bp

>T49145 NZ_CP009477:736114-736287 [Burkholderia pseudomallei MSHR2543]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGCCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT49145 WP_004525420.1 NZ_CP009477:736271-736555 [Burkholderia pseudomallei MSHR2543]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT49145 NZ_CP009477:736271-736555 [Burkholderia pseudomallei MSHR2543]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8