49140

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1639535..1640072 Replicon chromosome
Accession NZ_CP009473
Organism Burkholderia pseudomallei MSHR840

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BG19_RS06775 Protein ID WP_004549731.1
Coordinates 1639535..1639804 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BG19_RS06780 Protein ID WP_004525420.1
Coordinates 1639788..1640072 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG19_RS32680 1635124..1637409 + 2286 WP_082256913.1 S8 family peptidase -
BG19_RS40145 1638791..1638982 - 192 Protein_1339 hypothetical protein -
BG19_RS06775 1639535..1639804 + 270 WP_004549731.1 BrnT family toxin Toxin
BG19_RS06780 1639788..1640072 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BG19_RS06785 1640431..1641681 + 1251 WP_045603987.1 IS256 family transposase -
BG19_RS32700 1642075..1642458 + 384 Protein_1343 DDE-type integrase/transposase/recombinase -
BG19_RS32705 1642421..1642849 + 429 Protein_1344 IS110 family transposase -
BG19_RS06800 1643076..1643192 - 117 Protein_1345 IS3 family transposase -
BG19_RS06805 1643439..1644899 + 1461 WP_045603989.1 metallophosphoesterase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1631415..1648543 17128
- inside IScluster/Tn - - 1637639..1648543 10904


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T49140 WP_004549731.1 NZ_CP009473:1639535-1639804 [Burkholderia pseudomallei MSHR840]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T49140 NZ_CP009473:1639535-1639804 [Burkholderia pseudomallei MSHR840]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT49140 WP_004525420.1 NZ_CP009473:1639788-1640072 [Burkholderia pseudomallei MSHR840]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT49140 NZ_CP009473:1639788-1640072 [Burkholderia pseudomallei MSHR840]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References