Detailed information of TA system TA049128

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4270078..4270769
• Replicon: chromosome
• Accession: NZ_CP009461
• Organism: Klebsiella pneumoniae strain XH209

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A483YFJ1
• Locus tag: LQ47_RS21080
• Protein ID: WP_025987721.1
• Coordinates: 4270078..4270419 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LQ47_RS21085
• Protein ID: WP_038434619.1
• Coordinates: 4270443..4270769 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ47_RS21065	4267077..4268312	-	1236	WP_068854594.1	hypothetical protein	-
LQ47_RS21070	4268731..4269063	-	333	WP_074439534.1	hypothetical protein	-
LQ47_RS21075	4269130..4269963	-	834	WP_038434618.1	DUF4942 domain-containing protein	-
LQ47_RS21080	4270078..4270419	-	342	WP_025987721.1	TA system toxin CbtA family protein	Toxin
LQ47_RS21085	4270443..4270769	-	327	WP_038434619.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LQ47_RS21090	4270784..4271260	-	477	WP_038434581.1	DNA repair protein RadC	-
LQ47_RS21095	4271271..4271717	-	447	WP_038434582.1	antirestriction protein	-
LQ47_RS21100	4271941..4272630	-	690	WP_038434583.1	hypothetical protein	-
LQ47_RS21105	4272950..4273303	+	354	WP_038434584.1	hypothetical protein	-
LQ47_RS21110	4273340..4274230	-	891	WP_038434585.1	50S ribosome-binding GTPase	-
LQ47_RS21115	4274302..4275390	-	1089	WP_038434586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4267077..4286341	19264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12867.90 Da
• Isoelectric Point: 8.5195

>T49128 WP_025987721.1 NZ_CP009461:c4270419-4270078 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Nucleotide

• Length: 342 bp

>T49128 NZ_CP009461:c4270419-4270078 [Klebsiella pneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGACAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12304.06 Da
• Isoelectric Point: 6.9560

>AT49128 WP_038434619.1 NZ_CP009461:c4270769-4270443 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDVECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT49128 NZ_CP009461:c4270769-4270443 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCTAATTTTGGCGCAAGGTTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGAAAATTTGGTGACGTCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Structures

Toxin

• AlphaFold DB: A0A483YFJ1