Detailed information of TA system TA049111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4536745..4537298
• Replicon: chromosome
• Accession: NZ_CP009460
• Organism: Dickeya fangzhongdai strain ND14b

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LH89_RS19680
• Protein ID: WP_038667629.1
• Coordinates: 4536984..4537298 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: LH89_RS19675
• Protein ID: WP_038667626.1
• Coordinates: 4536745..4536981 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH89_RS19645	4531965..4532143	+	179	Protein_3896	helix-turn-helix domain-containing protein	-
LH89_RS19665	4533983..4534678	+	696	WP_047642943.1	hypothetical protein	-
LH89_RS19670	4535479..4536426	+	948	WP_038667623.1	DUF1738 domain-containing protein	-
LH89_RS19675	4536745..4536981	+	237	WP_038667626.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LH89_RS19680	4536984..4537298	+	315	WP_038667629.1	CcdB family protein	Toxin
LH89_RS19685	4537332..4538084	-	753	WP_038667631.1	hypothetical protein	-
LH89_RS19690	4538093..4539829	-	1737	WP_038667634.1	type IV secretory system conjugative DNA transfer family protein	-
LH89_RS19695	4540621..4540923	-	303	WP_038667638.1	conjugal transfer protein	-
LH89_RS19700	4540966..4541982	-	1017	WP_038667641.1	P-type DNA transfer ATPase VirB11	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4524450..4560569	36119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11549.50 Da
• Isoelectric Point: 7.9859

>T49111 WP_038667629.1 NZ_CP009460:4536984-4537298 [Dickeya fangzhongdai]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPERLVPVIRLTDGKEYAVMSHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGF

Nucleotide

• Length: 315 bp

>T49111 NZ_CP009460:4536984-4537298 [Dickeya fangzhongdai]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCGCCGACCGGAGCGCCTTGTCC
CCGTCATCAGGCTGACGGACGGCAAAGAATACGCCGTAATGTCCCACGAGCTGGCAAGTATCCCTGTCCAGGCTCTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGTACTCAGGTAAAGGCAGCAATAGATTTCCTCATTGACGGGTTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8990.03 Da
• Isoelectric Point: 6.4610

>AT49111 WP_038667626.1 NZ_CP009460:4536745-4536981 [Dickeya fangzhongdai]
MTGKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRYHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT49111 NZ_CP009460:4536745-4536981 [Dickeya fangzhongdai]
ATGACCGGGAAACAACGCAACACGCAGAGCGTGACCATGACAGTAGAACGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
CATTAATCTGAGTGCTACCCTGACAACCGCCCTGGATGCAGAGCTTCGCTATCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTCTCGCAGCACTAAATCGTTTTCATGATGAACAGGGCTGTTTCAGCGATGAATACAGGACGTTTTAA