Detailed information of TA system TA049108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1523559..1524184
• Replicon: chromosome
• Accession: NZ_CP009460
• Organism: Dickeya fangzhongdai strain ND14b

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0SD87
• Locus tag: LH89_RS06440
• Protein ID: WP_009114000.1
• Coordinates: 1523981..1524184 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LH89_RS06435
• Protein ID: WP_038661190.1
• Coordinates: 1523559..1523927 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH89_RS06420	1519307..1522453	+	3147	WP_038661184.1	multidrug efflux RND transporter permease subunit	-
LH89_RS06425	1522640..1522891	+	252	WP_038661187.1	type B 50S ribosomal protein L31	-
LH89_RS06430	1522907..1523050	+	144	WP_013316871.1	type B 50S ribosomal protein L36	-
LH89_RS06435	1523559..1523927	+	369	WP_038661190.1	Hha toxicity modulator TomB	Antitoxin
LH89_RS06440	1523981..1524184	+	204	WP_009114000.1	hemolysin expression modulator Hha	Toxin
LH89_RS06445	1524745..1525074	+	330	WP_038661197.1	MGMT family protein	-
LH89_RS06450	1525103..1525663	-	561	WP_038661200.1	YbaY family lipoprotein	-
LH89_RS06455	1525895..1526758	+	864	WP_038661203.1	acyl-CoA thioesterase II	-
LH89_RS06460	1526855..1528147	-	1293	WP_038661206.1	ammonium transporter AmtB	-
LH89_RS06465	1528190..1528528	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8133.51 Da
• Isoelectric Point: 8.8500

>T49108 WP_009114000.1 NZ_CP009460:1523981-1524184 [Dickeya fangzhongdai]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Nucleotide

• Length: 204 bp

>T49108 NZ_CP009460:1523981-1524184 [Dickeya fangzhongdai]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCCGCAGACCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTGCCGACCGCTGTGTGGAAATACGTTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14200.03 Da
• Isoelectric Point: 4.9092

>AT49108 WP_038661190.1 NZ_CP009460:1523559-1523927 [Dickeya fangzhongdai]
MDEYTPQHYDIAQLRFLCENLHDESIATLGDSSRGWVNDPTSAVNLQLNELIEHIAAFVVTYKIKYPHEAALCERVEKYL
DDTYILFSNYGINDSELQKWQKSKSQLFRMFSEKSICTVVKT

Nucleotide

• Length: 369 bp

>AT49108 NZ_CP009460:1523559-1523927 [Dickeya fangzhongdai]
ATGGATGAGTACACACCTCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGACAGCAGTCGTGGCTGGGTGAATGATCCGACCTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCGGCGTTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGCAATTACGGTATAAATGACTCCGAGTTGCAGAAATGGCAAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A1X3RNQ0