Detailed information of TA system TA049107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1052142..1052898
• Replicon: chromosome
• Accession: NZ_CP009460
• Organism: Dickeya fangzhongdai strain ND14b

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: LH89_RS04550
• Protein ID: WP_038660239.1
• Coordinates: 1052641..1052898 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: LH89_RS04545
• Protein ID: WP_038660236.1
• Coordinates: 1052142..1052630 (-)
• Length: 163 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH89_RS04520	1048080..1049807	+	1728	WP_038660219.1	hydrogenase large subunit	-
LH89_RS04525	1049818..1050387	+	570	WP_038660223.1	hydrogenase 4 subunit H	-
LH89_RS04530	1050384..1051175	+	792	WP_038660225.1	NADH-quinone oxidoreductase subunit B family protein	-
LH89_RS04535	1051172..1051570	+	399	WP_038660229.1	formate hydrogenlyase maturation HycH family protein	-
LH89_RS04540	1051563..1052060	+	498	WP_038660233.1	hydrogenase maturation peptidase HycI	-
LH89_RS04545	1052142..1052630	-	489	WP_038660236.1	DUF2442 domain-containing protein	Antitoxin
LH89_RS04550	1052641..1052898	-	258	WP_038660239.1	DUF4160 domain-containing protein	Toxin
LH89_RS04555	1053396..1053938	+	543	WP_013317225.1	4Fe-4S dicluster domain-containing protein	-
LH89_RS04560	1053973..1056123	+	2151	WP_038660243.1	formate dehydrogenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (79-157)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9994.57 Da
• Isoelectric Point: 8.9627

>T49107 WP_038660239.1 NZ_CP009460:c1052898-1052641 [Dickeya fangzhongdai]
MPEIDRLAGLIFHLYFYDNERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNNKRKMALAHIRAHREQLLSMWERAVVGR
NPGKL

Nucleotide

• Length: 258 bp

>T49107 NZ_CP009460:c1052898-1052641 [Dickeya fangzhongdai]
ATGCCTGAGATTGACAGACTGGCAGGGCTAATTTTCCATTTGTACTTTTACGATAATGAACGGCACCACTTGCCGCATGT
ACATGTCTGTTATGGCAACCATGAGCTGATCATCAGTATTCGTACTGGAACAGAGATCGAAGGATATCTGCCGAATAACA
AGCGCAAGATGGCGCTTGCGCATATCAGAGCTCATCGGGAACAACTGCTGAGTATGTGGGAGCGGGCGGTTGTGGGGAGA
AATCCGGGAAAATTGTGA

Antitoxin

Protein

• Length: 163 a.a.
• Molecular weight: 17940.47 Da
• Isoelectric Point: 6.6439

>AT49107 WP_038660236.1 NZ_CP009460:c1052630-1052142 [Dickeya fangzhongdai]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYQYASSPVFSNTSLFIKFGLLPDGALEWSPDIKISASELRKKGEYVSHTLA
VRENNFADVISRAFYDAVIENRPEILQAALKIAVEKHGNSTVAKEAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EK

Nucleotide

• Length: 489 bp

>AT49107 NZ_CP009460:c1052630-1052142 [Dickeya fangzhongdai]
ATGCATAAAATCGTGGAAGTTGACGTGATTGGCGACTTTTCGTTGCGCCTGACCTATGAAGATGGTTTGGTTTGCGACAT
CAATCTTTATCAATATGCCTCCAGCCCGGTATTTTCGAACACCAGCCTATTTATCAAGTTTGGGCTGCTGCCGGATGGCG
CGCTGGAATGGTCGCCGGATATTAAGATTTCCGCGTCGGAATTGCGGAAAAAAGGGGAGTATGTCAGCCACACGCTTGCG
GTGCGAGAAAACAACTTCGCTGATGTCATCTCTCGCGCCTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCGGCGTTGAAAATCGCGGTGGAAAAACATGGCAACAGCACCGTAGCCAAAGAGGCGGGGGCGAAAAGTCGCACCAGCC
TATATAAGTCGCTGAATAAAAATACCCATGTCAGCCTGGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAACTGGAA
GAGAAGTAA