Detailed information of TA system TA049067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4654996..4655639
• Replicon: chromosome
• Accession: NZ_CP009456
• Organism: Yersinia enterocolitica strain FORC_002

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LI89_RS21105
• Protein ID: WP_046051923.1
• Coordinates: 4654996..4655415 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A0E1NFG4
• Locus tag: LI89_RS21110
• Protein ID: WP_005161959.1
• Coordinates: 4655412..4655639 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LI89_RS21085	4650202..4651071	+	870	WP_046694358.1	formate dehydrogenase accessory sulfurtransferase FdhD	-
LI89_RS21090	4651198..4651821	+	624	WP_013650637.1	superoxide dismutase [Mn]	-
LI89_RS21095	4652141..4654090	+	1950	WP_046694357.1	methyl-accepting chemotaxis protein	-
LI89_RS21100	4654169..4654840	+	672	WP_046694356.1	6-N-hydroxylaminopurine resistance protein	-
LI89_RS21105	4654996..4655415	-	420	WP_046051923.1	type II toxin-antitoxin system VapC family toxin	Toxin
LI89_RS21110	4655412..4655639	-	228	WP_005161959.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LI89_RS21115	4655771..4656127	-	357	WP_005161962.1	YibL family ribosome-associated protein	-
LI89_RS21120	4656220..4657701	-	1482	WP_046695633.1	PLP-dependent aminotransferase family protein	-
LI89_RS21125	4657845..4658456	+	612	WP_046051925.1	LysE family translocator	-
LI89_RS21130	4658453..4659007	-	555	WP_005161972.1	MltR family transcriptional regulator	-
LI89_RS21135	4659104..4660264	-	1161	WP_019081443.1	mannitol-1-phosphate 5-dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-131)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15580.20 Da
• Isoelectric Point: 9.0596

>T49067 WP_046051923.1 NZ_CP009456:c4655415-4654996 [Yersinia enterocolitica]
MIKKLYMLDTNICSFIMRERPAELIIKLQQCIEHQNKIVVSAITYSEMRFGAIGKKASPKHNYLVDEFVKRLDAILPWDV
AAVDATTNIKVELAKQGTPIGGNDAAIAGHAVSAGAILVTNNIREFERVKKLRIEDWTQ

Nucleotide

• Length: 420 bp

>T49067 NZ_CP009456:c4655415-4654996 [Yersinia enterocolitica]
ATGATCAAAAAATTATATATGCTTGATACGAATATTTGTTCTTTTATTATGCGAGAACGACCAGCAGAATTAATTATAAA
ATTACAACAGTGTATTGAGCATCAGAATAAAATAGTTGTTTCAGCAATTACATACTCAGAAATGCGGTTCGGCGCTATAG
GTAAGAAAGCATCTCCAAAGCATAATTATTTAGTTGATGAATTTGTAAAAAGGTTAGATGCTATTTTACCGTGGGATGTC
GCAGCTGTTGATGCCACGACTAATATTAAAGTTGAGCTGGCAAAGCAGGGGACACCAATTGGTGGAAATGATGCCGCAAT
AGCCGGGCATGCAGTTTCTGCTGGAGCAATATTAGTAACTAATAATATTAGAGAGTTTGAACGAGTTAAAAAACTACGAA
TTGAAGATTGGACTCAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8786.91 Da
• Isoelectric Point: 4.1971

>AT49067 WP_005161959.1 NZ_CP009456:c4655639-4655412 [Yersinia enterocolitica]
MRTVSIFKNGQNRAIRLPKDMDFDGVTELEILRDGDSIILRPIRPTWVSFMDEDKADDDFLAERQDVIEEGRFEL

Nucleotide

• Length: 228 bp

>AT49067 NZ_CP009456:c4655639-4655412 [Yersinia enterocolitica]
ATGAGAACAGTATCTATTTTCAAAAATGGGCAGAATCGAGCAATTCGTTTGCCAAAAGATATGGATTTTGATGGCGTGAC
CGAGCTGGAAATACTCAGAGACGGTGATAGTATTATTTTACGACCCATACGCCCGACATGGGTATCATTCATGGATGAAG
ATAAGGCAGATGATGATTTTCTTGCCGAAAGACAGGATGTTATCGAAGAAGGACGCTTCGAGTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1NFG4