Detailed information of TA system TA049052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3309135..3309791
• Replicon: chromosome
• Accession: NZ_CP009455
• Organism: Pseudomonas cremoricolorata strain ND07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0Q0X232
• Locus tag: LK03_RS15095
• Protein ID: WP_038413159.1
• Coordinates: 3309612..3309791 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A089WVG4
• Locus tag: LK03_RS15090
• Protein ID: WP_038413158.1
• Coordinates: 3309135..3309560 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK03_RS15050	3304349..3305320	+	972	WP_038413150.1	hypothetical protein	-
LK03_RS15055	3305307..3306086	+	780	WP_038413151.1	hypothetical protein	-
LK03_RS15060	3306083..3306379	+	297	WP_038413152.1	DUF1364 domain-containing protein	-
LK03_RS15065	3306376..3306801	+	426	WP_038413153.1	VRR-NUC domain-containing protein	-
LK03_RS15070	3306798..3308096	+	1299	WP_038413154.1	tyrosine-type recombinase/integrase	-
LK03_RS15075	3308093..3308341	+	249	WP_038413155.1	hypothetical protein	-
LK03_RS15080	3308338..3308655	+	318	WP_038413156.1	hypothetical protein	-
LK03_RS15085	3308667..3309008	+	342	WP_038413157.1	hypothetical protein	-
LK03_RS15090	3309135..3309560	-	426	WP_038413158.1	transcriptional regulator	Antitoxin
LK03_RS15095	3309612..3309791	-	180	WP_038413159.1	type II toxin-antitoxin system HicA family toxin	Toxin
LK03_RS15100	3309887..3310213	+	327	WP_038413161.1	hypothetical protein	-
LK03_RS15105	3310210..3310539	+	330	WP_038413162.1	hypothetical protein	-
LK03_RS21995	3310543..3310947	+	405	WP_081951608.1	HNH endonuclease	-
LK03_RS15115	3311048..3311527	+	480	WP_038413164.1	phage terminase small subunit P27 family	-
LK03_RS15120	3311527..3313254	+	1728	WP_038413165.1	terminase large subunit	-
LK03_RS22330	3313254..3313418	+	165	WP_167334500.1	hypothetical protein	-
LK03_RS15125	3313411..3314721	+	1311	WP_038413166.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3241021..3334470	93449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6634.64 Da
• Isoelectric Point: 11.1475

>T49052 WP_038413159.1 NZ_CP009455:c3309791-3309612 [Pseudomonas cremoricolorata]
MKFSEFRRWLKAQGVTFEAGKGSHFKVTAPNGNRTTFADHGSKEMPEPTRKAIIKQLGL

Nucleotide

• Length: 180 bp

>T49052 NZ_CP009455:c3309791-3309612 [Pseudomonas cremoricolorata]
ATGAAGTTCAGCGAGTTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCAGGCAAAGGAAGCCACTTCAAAGT
CACCGCCCCGAACGGCAACAGGACAACGTTTGCGGACCACGGATCAAAGGAAATGCCCGAACCGACCCGCAAAGCGATCA
TTAAACAACTGGGGCTCTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15831.15 Da
• Isoelectric Point: 4.9621

>AT49052 WP_038413158.1 NZ_CP009455:c3309560-3309135 [Pseudomonas cremoricolorata]
MYNYAIRFEQDSTPGLAVFCRDLPELNSYGDDREHAISEAVDAIEVTLSIYVDQRRPIPESSEPLPGEQVIYLPAVTVAK
ILLWNTMMERGMRKADLRRLLGVHQSQGDRLVDFMHTSKMEQIEAALAALGKRLAVTVQAA

Nucleotide

• Length: 426 bp

>AT49052 NZ_CP009455:c3309560-3309135 [Pseudomonas cremoricolorata]
ATGTACAACTACGCAATCCGCTTCGAGCAAGACTCCACGCCGGGCCTGGCGGTGTTCTGCCGCGACCTTCCAGAGCTGAA
CAGCTACGGCGATGACCGGGAGCACGCGATAAGCGAGGCCGTCGACGCCATCGAAGTGACACTCTCGATCTATGTTGATC
AGCGCAGACCTATTCCAGAATCCAGCGAGCCACTTCCAGGGGAGCAGGTCATTTACCTGCCAGCCGTGACCGTGGCGAAG
ATCTTGCTTTGGAACACGATGATGGAACGTGGCATGCGTAAGGCCGACCTGCGCCGGTTGCTTGGCGTTCACCAGTCACA
AGGCGATCGACTGGTAGATTTCATGCACACATCGAAGATGGAGCAGATTGAAGCGGCTTTGGCAGCGCTTGGTAAACGGT
TGGCTGTCACGGTCCAAGCTGCATGA

Structures

Toxin

• AlphaFold DB: A0A0Q0X232

Antitoxin

• AlphaFold DB: A0A089WVG4