Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3682786..3683406 Replicon chromosome
Accession NZ_CP009454
Organism Pantoea rwandensis strain ND04

Toxin (Protein)


Gene name Hha Uniprot ID A0A0B1RA72
Locus tag LH22_RS16955 Protein ID WP_034828137.1
Coordinates 3683188..3683406 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2M9WAL2
Locus tag LH22_RS16950 Protein ID WP_034828139.1
Coordinates 3682786..3683163 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH22_RS16925 3679094..3679351 + 258 WP_038648520.1 type B 50S ribosomal protein L31 -
LH22_RS20355 3679366..3679506 + 141 WP_008103894.1 type B 50S ribosomal protein L36 -
LH22_RS16930 3679551..3680429 - 879 WP_038648522.1 metal ABC transporter substrate-binding protein -
LH22_RS16935 3680451..3681290 - 840 WP_038648524.1 metal ABC transporter permease -
LH22_RS16940 3681287..3681943 - 657 WP_034828143.1 ABC transporter ATP-binding protein -
LH22_RS16945 3682286..3682639 + 354 WP_034828141.1 hypothetical protein -
LH22_RS16950 3682786..3683163 + 378 WP_034828139.1 Hha toxicity modulator TomB Antitoxin
LH22_RS16955 3683188..3683406 + 219 WP_034828137.1 hemolysin expression modulator Hha Toxin
LH22_RS16965 3683807..3684118 + 312 WP_038648530.1 MGMT family protein -
LH22_RS16970 3684505..3685062 - 558 WP_038648532.1 YbaY family lipoprotein -
LH22_RS16975 3685267..3686130 + 864 WP_038648534.1 acyl-CoA thioesterase II -
LH22_RS16980 3686202..3687491 - 1290 WP_038648536.1 ammonium transporter AmtB -
LH22_RS16985 3687525..3687863 - 339 WP_007886947.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8584.94 Da        Isoelectric Point: 9.5023

>T49050 WP_034828137.1 NZ_CP009454:3683188-3683406 [Pantoea rwandensis]
MSNQALTKTDYLMRLRRCRSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKVPVSVWKFVR

Download         Length: 219 bp

>T49050 NZ_CP009454:3683188-3683406 [Pantoea rwandensis]
ATGAGCAACCAAGCCTTAACCAAAACAGACTATTTGATGCGACTGCGTCGTTGTCGCTCAATTGACACCCTTGAACGGGT
GATTGAAAAGAACAAGTACGAATTATCTGACAACGAGCTCGCGGTATTTTATTCTGCTGCCGATCATCGCCTTGCAGAAC
TGACGATGAATAAGCTTTACGATAAAGTACCGGTTTCTGTCTGGAAGTTCGTCCGCTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14723.38 Da        Isoelectric Point: 4.4069

>AT49050 WP_034828139.1 NZ_CP009454:3682786-3683163 [Pantoea rwandensis]
MDEYSPKRHDIAQLKFLCENLFDESMATLTDSHHGWVNDPTAESNLQLNDLIEHIASFTMNYKIKHVEDEALITQIDEYL
DDTFMLFSSYGINTQDLQRWQRSARRLFNLFAEECAFLQQPSHSF

Download         Length: 378 bp

>AT49050 NZ_CP009454:3682786-3683163 [Pantoea rwandensis]
ATGGACGAATACTCACCAAAACGGCATGATATTGCCCAGCTTAAATTCCTGTGTGAGAACCTGTTTGACGAAAGTATGGC
GACACTGACTGACAGCCATCACGGCTGGGTGAATGACCCTACTGCGGAAAGCAATTTACAGCTCAACGATTTGATTGAGC
ATATCGCGTCTTTCACGATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTACGCAAATCGATGAATATCTT
GACGATACCTTTATGCTGTTTAGCAGTTATGGAATCAATACTCAGGATCTTCAACGGTGGCAACGTTCAGCAAGACGCTT
ATTTAATCTGTTTGCCGAAGAGTGCGCTTTTCTCCAACAACCCAGCCATTCCTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B1RA72

Antitoxin

Source ID Structure
AlphaFold DB A0A2M9WAL2

References