Detailed information of TA system TA049042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2851939..2853467
• Replicon: chromosome
• Accession: NZ_CP009452
• Organism: Sphingopyxis sp. 113P3

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A0M4DBG3
• Locus tag: LH20_RS13930
• Protein ID: WP_053554729.1
• Coordinates: 2852157..2853467 (+)
• Length: 437 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: LH20_RS13925
• Protein ID: WP_053554728.1
• Coordinates: 2851939..2852154 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH20_RS13910	2848989..2850692	+	1704	WP_053554725.1	DUF885 family protein	-
LH20_RS13915	2850814..2851251	-	438	WP_144423579.1	hypothetical protein	-
LH20_RS13920	2851250..2851516	+	267	WP_144423580.1	hypothetical protein	-
LH20_RS22980	2851540..2851752	-	213	Protein_2746	DUF2384 domain-containing protein	-
LH20_RS13925	2851939..2852154	+	216	WP_053554728.1	helix-turn-helix transcriptional regulator	Antitoxin
LH20_RS13930	2852157..2853467	+	1311	WP_053554729.1	type II toxin-antitoxin system HipA family toxin	Toxin
LH20_RS13935	2853457..2854260	+	804	WP_053554730.1	aldehyde dehydrogenase family protein	-
LH20_RS13940	2854527..2855357	+	831	WP_053554731.1	alpha/beta hydrolase	-
LH20_RS13945	2855535..2857151	+	1617	WP_053554732.1	GMC family oxidoreductase N-terminal domain-containing protein	-
LH20_RS13950	2857233..2858219	-	987	WP_144423581.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (3-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-64)

Sequences

Toxin

Protein

• Length: 437 a.a.
• Molecular weight: 46600.02 Da
• Isoelectric Point: 5.6301

>T49042 WP_053554729.1 NZ_CP009452:2852157-2853467 [Sphingopyxis sp. 113P3]
VDLPVHYEDRLVATISADAQATRLAYADAWGSSADSFPVSLAMPIRPEPYEGEQVLPWLMNLLPEGEPLRAMTRALGAAP
GDALGLIAQTGNDLAGALSIAPQQPRGEPGYRPIPDDGALERIVEELPARPFLVGEDGVSMSLAGAQEKLPVTVIEGQLA
VPINGAPSTHILKPDNPRLPGSVQNEALCMVLARRIGLNVAPVTAGIAGQRSYLLVERYDRAGIGSNVRRLHQEDFCQAL
GRPPAAKYEFNGTGTRGPSIADMFALVRQHMTARDITRLLDAMIFNIAIGNVDSHAKNYSILLGPGAPQLAPLYDLMSGL
AWTNITQNHAQAIGGQRRGRHIYGRHWWRMAEAAGLAARGTVQRVEQVTARLLRELHAAVEEVAAMPAGSSMLKVFAKEI
EERATEVKAHAGQEGVPEVSGGVDEAPSLGGSAYDQ

Nucleotide

• Length: 1311 bp

>T49042 NZ_CP009452:2852157-2853467 [Sphingopyxis sp. 113P3]
GTGGATCTTCCGGTTCACTACGAAGACCGCTTGGTCGCTACGATCAGCGCCGACGCGCAGGCCACCCGCCTTGCCTATGC
GGATGCCTGGGGTTCATCCGCTGACAGCTTCCCGGTCTCGCTGGCGATGCCGATCCGGCCAGAGCCCTATGAGGGTGAAC
AGGTTCTTCCCTGGCTGATGAACCTTCTACCGGAAGGCGAACCGCTGCGCGCGATGACGCGGGCGCTCGGCGCGGCACCG
GGGGACGCACTCGGCCTGATCGCGCAGACCGGCAATGATCTGGCAGGCGCGCTCAGTATCGCGCCGCAGCAACCGCGCGG
CGAGCCCGGCTATCGTCCGATCCCGGATGACGGTGCACTTGAACGTATCGTCGAGGAACTGCCCGCGCGGCCATTCCTGG
TGGGCGAAGACGGCGTCTCGATGAGTCTTGCCGGCGCACAGGAAAAGCTGCCGGTGACCGTCATAGAAGGCCAGCTTGCG
GTGCCGATCAACGGCGCGCCGTCGACGCACATCCTCAAGCCCGACAATCCACGACTGCCCGGCAGCGTCCAAAATGAAGC
GCTCTGCATGGTTCTCGCCCGCCGGATCGGGCTCAATGTCGCGCCGGTGACGGCAGGCATAGCAGGGCAACGCAGCTACC
TGCTTGTCGAGCGCTATGATCGGGCAGGCATCGGCAGCAATGTTCGCCGTCTGCATCAGGAGGATTTCTGCCAGGCCCTC
GGCCGGCCGCCTGCCGCGAAGTACGAGTTCAACGGCACCGGCACGCGCGGCCCTTCGATCGCAGACATGTTCGCCCTGGT
CCGCCAGCATATGACCGCGCGCGACATCACCCGACTACTGGACGCAATGATCTTCAATATTGCGATCGGCAACGTCGATT
CGCATGCCAAGAACTACTCGATCCTGCTCGGCCCGGGCGCGCCGCAGCTTGCCCCGCTCTATGACCTGATGTCCGGCCTC
GCTTGGACGAACATCACCCAGAATCACGCCCAGGCCATCGGCGGCCAACGCCGCGGCCGCCACATCTACGGCCGCCACTG
GTGGCGCATGGCCGAAGCCGCAGGGCTGGCAGCGCGAGGAACGGTGCAGCGGGTAGAACAGGTGACGGCACGGTTGCTGC
GAGAGCTACACGCAGCGGTCGAAGAGGTGGCAGCCATGCCAGCAGGTTCGTCGATGCTGAAGGTCTTCGCAAAGGAGATC
GAAGAGCGGGCAACCGAAGTAAAGGCGCACGCCGGGCAGGAAGGTGTGCCAGAAGTAAGCGGAGGCGTGGATGAGGCACC
TTCGTTGGGAGGCTCAGCATATGATCAATAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7219.38 Da
• Isoelectric Point: 10.4692

>AT49042 WP_053554728.1 NZ_CP009452:2851939-2852154 [Sphingopyxis sp. 113P3]
MIDNAIEFGAAVASARKTLGLTQRELALAINSGERFIVDLEAGKPTAQLGKALAAARAVGLRLTATGISPA

Nucleotide

• Length: 216 bp

>AT49042 NZ_CP009452:2851939-2852154 [Sphingopyxis sp. 113P3]
ATGATCGACAACGCAATCGAATTCGGCGCAGCCGTCGCATCCGCTCGCAAGACCCTTGGCCTTACCCAGCGGGAGCTGGC
GCTCGCAATCAACAGCGGCGAACGCTTCATTGTCGATCTGGAAGCAGGAAAGCCCACTGCGCAACTCGGTAAGGCCCTCG
CCGCCGCTAGGGCTGTCGGCTTGCGGCTCACCGCAACCGGCATTTCGCCGGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0M4DBG3