Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 2427209..2427789 Replicon chromosome
Accession NZ_CP009452
Organism Sphingopyxis sp. 113P3

Toxin (Protein)


Gene name PumA Uniprot ID A0A1T5AHV2
Locus tag LH20_RS11855 Protein ID WP_053554368.1
Coordinates 2427493..2427789 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1T5AHP0
Locus tag LH20_RS11850 Protein ID WP_053554367.1
Coordinates 2427209..2427490 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH20_RS11830 2422609..2422905 - 297 WP_200905492.1 hypothetical protein -
LH20_RS11835 2422989..2424311 - 1323 WP_053554365.1 recombinase family protein -
LH20_RS11840 2424308..2424721 - 414 WP_200905493.1 DUF2924 domain-containing protein -
LH20_RS23530 2425255..2425752 + 498 WP_158501132.1 hypothetical protein -
LH20_RS11845 2426476..2426958 + 483 WP_053554366.1 hypothetical protein -
LH20_RS11850 2427209..2427490 - 282 WP_053554367.1 putative addiction module antidote protein Antitoxin
LH20_RS11855 2427493..2427789 - 297 WP_053554368.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LH20_RS22945 2427788..2428102 + 315 WP_158501133.1 HNH endonuclease -
LH20_RS23535 2428209..2429927 + 1719 WP_158501134.1 ParB N-terminal domain-containing protein -
LH20_RS11865 2429964..2430380 + 417 WP_053553102.1 IS66 family insertion sequence hypothetical protein -
LH20_RS11870 2430377..2430724 + 348 WP_053554370.1 IS66 family insertion sequence element accessory protein TnpB -
LH20_RS11875 2430788..2432416 + 1629 WP_053554371.1 IS66 family transposase -
LH20_RS11880 2432416..2432709 + 294 WP_053554372.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2385654..2429927 44273


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-95)

Antitoxin

Uncharacterized protein HI1420

(5-92)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 10895.57 Da        Isoelectric Point: 9.0454

>T49041 WP_053554368.1 NZ_CP009452:c2427789-2427493 [Sphingopyxis sp. 113P3]
MIEVRQTEEFAQWLASLRDGVAAKRIKQRIARVQIGLFGDVKPVGEGLSELRIDHGPGYRVYFARRGDILVILLCGGDKG
SQARDIARAKAMAEQLED

Download         Length: 297 bp

>T49041 NZ_CP009452:c2427789-2427493 [Sphingopyxis sp. 113P3]
ATGATTGAAGTCCGCCAGACGGAAGAATTCGCCCAATGGCTGGCATCGCTGCGGGATGGTGTAGCTGCCAAACGGATCAA
GCAGCGCATCGCCCGTGTCCAGATCGGACTATTTGGCGATGTCAAACCGGTTGGCGAAGGCCTCAGTGAACTGCGGATCG
ATCATGGACCAGGCTACCGCGTCTATTTTGCGCGGCGGGGTGACATTCTGGTGATCCTGCTGTGCGGCGGTGACAAAGGT
AGCCAAGCCCGCGACATTGCCCGCGCCAAGGCGATGGCTGAACAACTGGAGGACTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 9401.85 Da        Isoelectric Point: 4.9622

>AT49041 WP_053554367.1 NZ_CP009452:c2427490-2427209 [Sphingopyxis sp. 113P3]
MALKTLPFDAADALDTPEAQAELLAEALASGDAKVVTAALGMIARAKGMSQMARDAGISREAIYRATGPDGNPTLSTLMA
IVRSAGLKLSAAA

Download         Length: 282 bp

>AT49041 NZ_CP009452:c2427490-2427209 [Sphingopyxis sp. 113P3]
ATGGCTCTTAAAACTCTTCCCTTCGATGCCGCTGACGCCCTCGACACCCCTGAGGCGCAGGCCGAACTGCTTGCCGAGGC
TCTTGCCAGTGGCGATGCGAAGGTCGTGACCGCTGCCCTTGGCATGATTGCCCGTGCAAAAGGCATGAGCCAGATGGCTC
GTGACGCTGGCATCAGCCGCGAAGCGATTTACCGCGCCACTGGTCCTGACGGTAATCCAACCCTTTCCACGCTGATGGCT
ATCGTCCGCTCGGCAGGGCTGAAGCTTTCGGCGGCAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1T5AHV2

Antitoxin

Source ID Structure
AlphaFold DB A0A1T5AHP0

References