Detailed information of TA system TA049006

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2119364..2120022
• Replicon: chromosome
• Accession: NZ_CP009450
• Organism: Pluralibacter gergoviae strain FB2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A806ZJD4
• Locus tag: LG71_RS25835
• Protein ID: WP_071883340.1
• Coordinates: 2119364..2119684 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A806NTI2
• Locus tag: LG71_RS09970
• Protein ID: WP_043082396.1
• Coordinates: 2119705..2120022 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG71_RS09955	2115252..2115667	-	416	Protein_1998	transposase	-
LG71_RS25830	2115853..2117625	+	1773	WP_071883339.1	AAA family ATPase	-
LG71_RS09960	2117793..2118833	+	1041	WP_043082394.1	IS481 family transposase	-
LG71_RS09965	2118892..2119038	-	147	Protein_2001	integrase	-
LG71_RS25835	2119364..2119684	-	321	WP_071883340.1	TA system toxin CbtA family protein	Toxin
LG71_RS09970	2119705..2120022	-	318	WP_043082396.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LG71_RS25840	2120041..2120262	-	222	WP_000691995.1	DUF987 domain-containing protein	-
LG71_RS09975	2120271..2120747	-	477	WP_043082397.1	RadC family protein	-
LG71_RS09980	2120763..2121221	-	459	WP_043082398.1	antirestriction protein	-
LG71_RS25845	2121302..2121538	-	237	WP_071883341.1	DUF905 domain-containing protein	-
LG71_RS09985	2121616..2122026	-	411	WP_043082399.1	hypothetical protein	-
LG71_RS09990	2122116..2122730	-	615	WP_043082400.1	hypothetical protein	-
LG71_RS09995	2122727..2123431	-	705	WP_043082401.1	WYL domain-containing protein	-
LG71_RS10000	2123633..2124466	-	834	WP_043082402.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2111164..2161845	50681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-104)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12032.98 Da
• Isoelectric Point: 7.1339

>T49006 WP_071883340.1 NZ_CP009450:c2119684-2119364 [Pluralibacter gergoviae]
MKTLPATTLRAAKPCPSPVIVWQTLLSCLLDQHYGLTLNDTPFSDETVIKEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPFITAVDILKARRAMREMRT

Nucleotide

• Length: 321 bp

>T49006 NZ_CP009450:c2119684-2119364 [Pluralibacter gergoviae]
ATGAAAACTCTACCTGCAACGACTTTGCGGGCGGCTAAACCATGCCCGTCACCAGTGATCGTATGGCAAACACTTCTTTC
ATGCCTGCTGGATCAGCACTATGGCCTGACACTGAATGACACGCCATTCAGTGATGAAACTGTGATTAAGGAACATATCG
ATGCCGGTATCTCTTTAGCCGATGCTGTTAATTTTCTGGTGGAAAAATACGAACTGGTTCGCATCGATCGTAAGGGGTTT
AGCTGGCAAGAACAGTCGCCTTTTATTACTGCTGTAGACATTCTAAAAGCCAGACGGGCTATGAGGGAAATGAGAACATA
A

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12053.65 Da
• Isoelectric Point: 6.6251

>AT49006 WP_043082396.1 NZ_CP009450:c2120022-2119705 [Pluralibacter gergoviae]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDVECRKLDETFPHFISQMESMLTTDELNPRHTHCVILYH
NGFTCEADTLGSCGYVYITVYPTQR

Nucleotide

• Length: 318 bp

>AT49006 NZ_CP009450:c2120022-2119705 [Pluralibacter gergoviae]
ATGAGTAACATCACATGGGGCCTGCAGCGGGATATCACACCGCGCCTGGGAGCCCGCCTTGTGCAGGAGGGTAACCGGCT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGTAAATTCAGTGACGTCGAATGCCGGAAGCTAGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAGTCGATGCTGACCACCGATGAACTGAATCCCCGCCATACCCACTGCGTCATCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCACCGTTTATCCCACTCAACGATAA

Structures

Toxin

• AlphaFold DB: A0A806ZJD4

Antitoxin

• AlphaFold DB: A0A806NTI2