Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 944834..945450 Replicon chromosome
Accession NZ_CP009450
Organism Pluralibacter gergoviae strain FB2

Toxin (Protein)


Gene name vapC Uniprot ID A0A1Y4XBI2
Locus tag LG71_RS04435 Protein ID WP_043081487.1
Coordinates 945079..945450 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1S2BMV8
Locus tag LG71_RS04430 Protein ID WP_043081486.1
Coordinates 944834..945076 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LG71_RS04405 941295..941885 + 591 WP_043081481.1 glucose-1-phosphatase -
LG71_RS04410 941890..942753 + 864 WP_043081482.1 virulence factor BrkB family protein -
LG71_RS04415 942750..943187 + 438 WP_043081483.1 D-tyrosyl-tRNA(Tyr) deacylase -
LG71_RS04420 943220..944161 + 942 WP_043081484.1 fatty acid biosynthesis protein FabY -
LG71_RS04425 944407..944697 + 291 WP_043081485.1 helix-turn-helix domain-containing protein -
LG71_RS04430 944834..945076 + 243 WP_043081486.1 ribbon-helix-helix protein, CopG family Antitoxin
LG71_RS04435 945079..945450 + 372 WP_043081487.1 type II toxin-antitoxin system VapC family toxin Toxin
LG71_RS04440 945478..946407 - 930 WP_043081488.1 formate dehydrogenase accessory protein FdhE -
LG71_RS04445 946679..947314 - 636 WP_043081489.1 formate dehydrogenase cytochrome b556 subunit -
LG71_RS04450 947311..948213 - 903 WP_043081490.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(16-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13444.46 Da        Isoelectric Point: 9.5889

>T48999 WP_043081487.1 NZ_CP009450:945079-945450 [Pluralibacter gergoviae]
MDGPAVFDTNILIDLFNNRSEAADAIEQRGPHRAVSLVTWMEVMAGAKKYGQEARTAGVFSLFEVVDINKEIARRSVSLR
ATYGMKLPDAIILATAQARGSALITRNTKDFAGIPGVITPYRF

Download         Length: 372 bp

>T48999 NZ_CP009450:945079-945450 [Pluralibacter gergoviae]
ATGGATGGCCCGGCCGTTTTCGATACTAATATCCTGATCGATCTCTTCAACAACCGCAGCGAGGCCGCCGATGCGATTGA
GCAACGCGGCCCGCACCGGGCGGTAAGTCTGGTGACCTGGATGGAGGTCATGGCGGGGGCGAAAAAGTACGGACAGGAGG
CCAGAACCGCTGGCGTGTTCAGCCTGTTCGAAGTCGTTGATATCAATAAAGAGATTGCCAGGCGCAGCGTCTCGCTGAGG
GCTACCTACGGCATGAAGCTCCCCGACGCCATTATACTGGCCACTGCGCAGGCGCGCGGCAGCGCGCTCATCACTCGCAA
TACCAAAGACTTCGCCGGTATTCCCGGCGTTATCACACCCTACCGGTTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9354.63 Da        Isoelectric Point: 4.4247

>AT48999 WP_043081486.1 NZ_CP009450:944834-945076 [Pluralibacter gergoviae]
MSMILETDVARILLDVSDDVIRRLDGLKQLRDRPRAELLREAIEQYLDRQSPSVLRDALGLWSDGGEDGLVYERKLREEW

Download         Length: 243 bp

>AT48999 NZ_CP009450:944834-945076 [Pluralibacter gergoviae]
ATGAGTATGATTTTGGAGACGGACGTGGCAAGAATCCTTCTTGATGTGTCTGATGACGTCATCAGGCGGCTTGATGGTCT
GAAGCAACTGAGGGACAGGCCGCGCGCGGAGCTGCTGCGCGAAGCGATAGAGCAGTATCTCGACAGGCAAAGCCCGTCGG
TGCTCAGAGACGCGCTCGGGCTGTGGAGCGACGGCGGGGAAGATGGCTTGGTTTACGAGCGCAAACTGCGCGAGGAGTGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y4XBI2

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2BMV8

References