Detailed information of TA system TA048996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 136371..137143
• Replicon: plasmid pBIN_1
• Accession: NZ_CP009433
• Organism: Burkholderia glumae LMG 2196 = ATCC 33617

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: KS03_RS29285
• Protein ID: WP_080942736.1
• Coordinates: 136649..137143 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2J9HHX2
• Locus tag: KS03_RS01290
• Protein ID: WP_045678699.1
• Coordinates: 136371..136652 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KS03_RS01270	133192..133911	+	720	WP_045678698.1	hypothetical protein	-
KS03_RS01275	133958..135037	+	1080	WP_017432519.1	MBL fold metallo-hydrolase	-
KS03_RS01280	135194..135475	-	282	WP_042967384.1	virulence factor	-
KS03_RS01285	135726..136183	-	458	Protein_118	transposase	-
KS03_RS01290	136371..136652	+	282	WP_045678699.1	DUF1778 domain-containing protein	Antitoxin
KS03_RS29285	136649..137143	+	495	WP_080942736.1	GNAT family N-acetyltransferase	Toxin
KS03_RS29290	137236..137706	+	471	WP_080942737.1	ProQ activator of osmoprotectant transporter prop	-
KS03_RS01300	137796..140717	-	2922	WP_017432540.1	conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..196020	196020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-84)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17493.08 Da
• Isoelectric Point: 8.6141

>T48996 WP_080942736.1 NZ_CP009433:136649-137143 [Burkholderia glumae LMG 2196 = ATCC 33617]
MTGWLAPEPLGDAHGLDDFDSGVASLDTWLKRRAFANQRSGASRTFVATRDGRVGAYYALASGAVTLEVAPGRFRRNMPD
PIPVVVLGRLAVDRAHQGNGVGRALVRDVGLRVLHAAAAIGIRGLIVHALTDSAKAFYERVGFEASPIDPMLLLITLADL
EHAL

Nucleotide

• Length: 495 bp

>T48996 NZ_CP009433:136649-137143 [Burkholderia glumae LMG 2196 = ATCC 33617]
ATGACGGGCTGGCTCGCGCCCGAACCGCTCGGCGACGCGCATGGACTCGACGATTTCGATTCCGGCGTCGCGAGCCTCGA
CACGTGGCTCAAACGTCGTGCATTTGCCAATCAGCGAAGCGGTGCGTCGCGGACCTTTGTGGCGACGCGAGACGGTCGGG
TGGGGGCGTACTACGCACTCGCGTCCGGCGCGGTTACGCTCGAGGTCGCACCGGGCCGCTTCCGACGCAACATGCCCGAT
CCGATACCCGTGGTTGTGCTCGGGCGTCTAGCCGTCGATCGCGCCCATCAGGGCAATGGAGTTGGGCGTGCGCTGGTACG
CGATGTCGGTTTGCGTGTACTGCACGCCGCAGCGGCCATCGGAATTCGCGGCCTGATTGTCCATGCGCTCACGGATTCGG
CCAAGGCGTTTTACGAGCGGGTGGGCTTTGAGGCGTCGCCAATCGATCCGATGCTGCTCCTGATCACGCTGGCCGACCTT
GAACACGCCTTATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10112.39 Da
• Isoelectric Point: 7.0104

>AT48996 WP_045678699.1 NZ_CP009433:136371-136652 [Burkholderia glumae LMG 2196 = ATCC 33617]
MTSTTPRDTLNLRIRPDDRSLIDRAAAAAHKTRTDFVLDAARAAAEQTLLDQVVLSAEPQAYAAFLARLDAPAQPNAALR
QTMQTRAPWDPAA

Nucleotide

• Length: 282 bp

>AT48996 NZ_CP009433:136371-136652 [Burkholderia glumae LMG 2196 = ATCC 33617]
ATGACCTCGACCACCCCACGCGACACCCTGAACCTACGCATTCGTCCTGACGATCGCAGCTTGATTGACCGCGCCGCAGC
GGCCGCCCACAAAACGCGTACCGACTTCGTCTTGGACGCAGCGCGGGCCGCCGCCGAACAGACCTTGCTCGACCAGGTTG
TTCTGAGCGCTGAGCCGCAGGCCTACGCGGCCTTCCTGGCGCGCCTGGATGCGCCCGCCCAACCGAATGCGGCATTGCGT
CAGACCATGCAGACGCGCGCGCCCTGGGACCCCGCCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2J9HHX2