Detailed information of TA system TA048993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 4468049..4468676
• Replicon: chromosome
• Accession: NZ_CP009429
• Organism: Sphingopyxis macrogoltabida strain 203

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LH19_RS21670
• Protein ID: WP_054731804.1
• Coordinates: 4468049..4468441 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0N7I258
• Locus tag: LH19_RS21675
• Protein ID: WP_054589308.1
• Coordinates: 4468446..4468676 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH19_RS21645	4463249..4464007	+	759	WP_054731800.1	arsenical resistance protein ArsH	-
LH19_RS21650	4464495..4465811	+	1317	WP_054731801.1	site-specific integrase	-
LH19_RS21655	4465885..4466211	-	327	WP_054734108.1	DUF736 family protein	-
LH19_RS28845	4466391..4466741	+	351	WP_145923557.1	hypothetical protein	-
LH19_RS21665	4467093..4467551	-	459	WP_054731803.1	MucR family transcriptional regulator	-
LH19_RS21670	4468049..4468441	-	393	WP_054731804.1	PIN domain-containing protein	Toxin
LH19_RS21675	4468446..4468676	-	231	WP_054589308.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LH19_RS28180	4469008..4469780	+	773	WP_145923346.1	IS5 family transposase	-
LH19_RS21690	4470893..4471297	-	405	WP_145923558.1	helix-turn-helix domain-containing protein	-
LH19_RS28855	4472304..4472963	-	660	WP_145923559.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4259348..4494685	235337
	-	flank	IS/Tn	-	-	4469008..4469376	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-113)

Antitoxin

• SpoVT-AbrB domain (5-32)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14273.22 Da
• Isoelectric Point: 4.2178

>T48993 WP_054731804.1 NZ_CP009429:c4468441-4468049 [Sphingopyxis macrogoltabida]
MPVFLDTNILIYAFSDDPRTSTAEKLLSDGGEISVQVLNEFSNVARKKLQFDWQHLGEALGIVRGLASRVNPLNVETYIE
GIALAERYGISIYDGLIVASAIQAGCDILYSEDMHDGLGIGEQLRIVNPF

Nucleotide

• Length: 393 bp

>T48993 NZ_CP009429:c4468441-4468049 [Sphingopyxis macrogoltabida]
ATGCCCGTCTTTCTCGATACGAATATCCTCATCTACGCGTTCAGCGACGATCCGCGCACCAGCACAGCTGAGAAACTCTT
GTCGGACGGGGGCGAAATCAGCGTTCAAGTTCTGAACGAATTCTCGAACGTCGCCCGGAAGAAACTTCAGTTCGATTGGC
AACACCTCGGCGAAGCGCTGGGTATCGTTCGGGGTCTCGCAAGCCGCGTTAATCCCCTCAACGTAGAAACCTATATCGAG
GGGATCGCACTAGCGGAGCGATACGGGATCTCAATTTATGATGGGCTTATCGTTGCGTCCGCAATTCAAGCAGGATGCGA
CATACTATATTCGGAAGACATGCATGACGGACTGGGAATCGGTGAACAGTTGAGGATCGTGAATCCGTTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8887.18 Da
• Isoelectric Point: 8.9114

>AT48993 WP_054589308.1 NZ_CP009429:c4468676-4468446 [Sphingopyxis macrogoltabida]
MQLARWGNSLALRIPASIVELLGLKEGDDIEVNVAGTHRFEIDRDRSREKLVERMRMLRKPLPAGWRFDRDDANER

Nucleotide

• Length: 231 bp

>AT48993 NZ_CP009429:c4468676-4468446 [Sphingopyxis macrogoltabida]
ATGCAGCTCGCGCGCTGGGGAAATAGCCTCGCCCTTCGGATACCGGCATCCATCGTCGAATTGCTTGGTCTCAAGGAAGG
CGACGATATTGAGGTCAATGTCGCCGGGACTCACCGTTTCGAGATCGATCGGGATAGGTCTCGCGAGAAGCTGGTGGAAC
GCATGCGCATGTTGCGCAAGCCTTTGCCTGCCGGTTGGCGCTTTGATCGCGACGACGCCAACGAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0N7I258