Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1942143..1942732 Replicon chromosome
Accession NZ_CP009429
Organism Sphingopyxis macrogoltabida strain 203

Toxin (Protein)


Gene name vapC Uniprot ID A0A0P0DHD6
Locus tag LH19_RS09695 Protein ID WP_054727421.1
Coordinates 1942143..1942511 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0P0DB65
Locus tag LH19_RS09700 Protein ID WP_054727423.1
Coordinates 1942508..1942732 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH19_RS09680 1937311..1938488 + 1178 WP_145923348.1 IS3-like element ISSpma1 family transposase -
LH19_RS09685 1938550..1939221 - 672 WP_054727417.1 HAD family phosphatase -
LH19_RS28585 1939459..1940172 - 714 WP_145923402.1 hypothetical protein -
LH19_RS09690 1940302..1942125 - 1824 WP_054727419.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
LH19_RS09695 1942143..1942511 - 369 WP_054727421.1 PIN domain-containing protein Toxin
LH19_RS09700 1942508..1942732 - 225 WP_054727423.1 ribbon-helix-helix domain-containing protein Antitoxin
LH19_RS09705 1942763..1943227 - 465 WP_054727426.1 GNAT family N-acetyltransferase -
LH19_RS09710 1943229..1943546 - 318 WP_054727428.1 hypothetical protein -
LH19_RS09715 1943543..1944091 - 549 WP_054727430.1 GNAT family N-acetyltransferase -
LH19_RS09720 1944096..1945463 - 1368 WP_054727431.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
LH19_RS09725 1945511..1946173 + 663 WP_054727433.1 HAD-IA family hydrolase -
LH19_RS29175 1946170..1946496 - 327 WP_201258442.1 alpha/beta hydrolase -
LH19_RS29180 1946516..1946893 - 378 WP_201258441.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-105)

Antitoxin

Ribbon-helix-helix protein, CopG

(7-38)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13094.19 Da        Isoelectric Point: 9.1636

>T48992 WP_054727421.1 NZ_CP009429:c1942511-1942143 [Sphingopyxis macrogoltabida]
MIEAQFDSDILIDALNGVEAARSEIRRAGRKSVSRISWTEVMSAADPASVKAVEAFLGCFAIEEVGDAVARRAAALRAER
KGLTMADAFVLATAQISGRILVTRNIKVFPASMPGIRVPYTL

Download         Length: 369 bp

>T48992 NZ_CP009429:c1942511-1942143 [Sphingopyxis macrogoltabida]
ATGATCGAGGCGCAGTTCGACAGCGATATCCTCATCGACGCGCTGAACGGCGTCGAGGCGGCGCGCAGCGAGATTCGCCG
CGCCGGACGCAAGAGCGTCAGCCGGATCAGCTGGACCGAGGTGATGTCGGCCGCCGACCCCGCGTCGGTGAAGGCGGTCG
AGGCCTTTCTCGGCTGTTTCGCGATCGAGGAGGTCGGCGATGCGGTGGCCCGACGCGCCGCTGCGTTGAGGGCCGAACGC
AAGGGCTTGACGATGGCCGACGCCTTCGTCTTAGCAACCGCGCAAATATCGGGCCGCATCCTCGTTACGCGCAATATCAA
GGTGTTTCCGGCATCGATGCCGGGTATCCGCGTTCCCTACACGCTCTAG

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8384.38 Da        Isoelectric Point: 6.2939

>AT48992 WP_054727423.1 NZ_CP009429:c1942732-1942508 [Sphingopyxis macrogoltabida]
MRFLADIPDDDVQWLDALAASQGVSRAELMRRAVSAYRADISGDAIDNGFGIWRSRDDIGDGLKYQRRLRGKRE

Download         Length: 225 bp

>AT48992 NZ_CP009429:c1942732-1942508 [Sphingopyxis macrogoltabida]
ATGCGTTTCCTTGCCGATATTCCCGATGACGATGTCCAGTGGCTAGATGCGCTCGCGGCGTCGCAGGGCGTGTCGCGCGC
CGAGCTGATGCGCCGCGCGGTGAGCGCGTACCGCGCCGACATATCGGGCGACGCGATCGACAATGGGTTCGGCATATGGC
GGTCGCGTGACGATATCGGCGACGGCCTGAAATATCAGCGCCGCCTCAGGGGCAAGCGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P0DHD6

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0DB65

References