Detailed information of TA system TA048991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1243609..1244214
• Replicon: chromosome
• Accession: NZ_CP009428
• Organism: Paenibacillus odorifer strain DSM 15391

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1R0YSQ6
• Locus tag: PODO_RS05310
• Protein ID: WP_036686777.1
• Coordinates: 1243864..1244214 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PODO_RS05305
• Protein ID: WP_154117276.1
• Coordinates: 1243609..1243860 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PODO_RS05285	1238998..1240383	+	1386	WP_038569043.1	helix-turn-helix transcriptional regulator	-
PODO_RS05290	1240529..1240702	+	174	WP_094903681.1	aspartyl-phosphate phosphatase Spo0E family protein	-
PODO_RS05295	1240908..1241993	+	1086	WP_036686774.1	outer membrane lipoprotein carrier protein LolA	-
PODO_RS05300	1242182..1243363	+	1182	WP_036686775.1	alanine racemase	-
PODO_RS05305	1243609..1243860	+	252	WP_154117276.1	ribbon-helix-helix protein, CopG family	Antitoxin
PODO_RS05310	1243864..1244214	+	351	WP_036686777.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PODO_RS05315	1244422..1244979	+	558	WP_051491479.1	TetR/AcrR family transcriptional regulator	-
PODO_RS05320	1245151..1246350	+	1200	WP_038569046.1	tetracycline resistance MFS efflux pump	-
PODO_RS05325	1246590..1248752	+	2163	WP_174890051.1	RNA-binding transcriptional accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12778.71 Da
• Isoelectric Point: 5.1284

>T48991 WP_036686777.1 NZ_CP009428:1243864-1244214 [Paenibacillus odorifer]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T48991 NZ_CP009428:1243864-1244214 [Paenibacillus odorifer]
TTGATCGTTAAACGTGGCGATGTTTTTTTTGCTGATCTTTCGCCGGTTGTAGGTTCTGAACAAGGCGGAGTAAGGCCAGT
TCTGGTCATCCAGAACGATATTGGCAATCGCTTTAGCCCGACGGTAATTGTGGCTGCAATCACAGCCCAAATCCAGAAGG
CTAAGTTACCTACACATGTGGAAATTGACGCAGCTGCTCATGGCTTTGACAGAGATTCTGTTATTTTGCTGGAACAGGTT
AGGACGATTGACAAACAACGCTTAACTGACAAGATCACCCACCTTGATGATGAAACGATGAAAAAGGTGGATGAATCGCT
GCAAATCAGCCTTGGTCTGATTGATTTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9487.94 Da
• Isoelectric Point: 5.1184

>AT48991 WP_154117276.1 NZ_CP009428:1243609-1243860 [Paenibacillus odorifer]
MISLPDHLLQEVDGIVQLENSNRSELIRQAMKLYLSERRKRSIRESMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT48991 NZ_CP009428:1243609-1243860 [Paenibacillus odorifer]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAAGTGGACGGAATTGTTCAATTGGAGAATTCCAACCGGAGTGAATTGAT
TAGGCAGGCCATGAAGCTGTACTTGAGCGAACGGAGGAAACGTTCCATCCGGGAGTCCATGCAGCGCGGCTACATGGAAA
TGGCTAAGATCAACTTGACCATGGCATGCGAGGCTTTCCTCGCGGAGGAAGATGCAGACAGCACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1R0YSQ6