Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3144835..3145439 Replicon chromosome
Accession NZ_CP009427
Organism Mycobacterium tuberculosis strain 96121

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag HR37_RS15025 Protein ID WP_003414492.1
Coordinates 3144835..3145227 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag HR37_RS15030 Protein ID WP_003414495.1
Coordinates 3145224..3145439 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR37_RS15000 3140075..3140449 - 375 WP_003414296.1 type III-A CRISPR-associated protein Csm2 -
HR37_RS15005 3140446..3142884 - 2439 WP_003904061.1 type III-A CRISPR-associated protein Cas10/Csm1 -
HR37_RS15010 3142881..3143669 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
HR37_RS15015 3144003..3144548 - 546 WP_003908074.1 DUF1802 family protein -
HR37_RS15020 3144545..3144814 - 270 WP_003912856.1 DUF2277 family protein -
HR37_RS15025 3144835..3145227 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
HR37_RS15030 3145224..3145439 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HR37_RS15035 3145486..3146235 + 750 WP_003908075.1 enoyl-CoA hydratase -
HR37_RS15040 3146314..3147396 - 1083 WP_003908076.1 ABC transporter ATP-binding protein -
HR37_RS15045 3147389..3148699 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
HR37_RS15050 3148702..3149529 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
HR37_RS15055 3149526..3150437 - 912 WP_003904073.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T48983 WP_003414492.1 NZ_CP009427:c3145227-3144835 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T48983 NZ_CP009427:c3145227-3144835 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT48983 WP_003414495.1 NZ_CP009427:c3145439-3145224 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT48983 NZ_CP009427:c3145439-3145224 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References