Detailed information of TA system TA048982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3119993..3120563
• Replicon: chromosome
• Accession: NZ_CP009427
• Organism: Mycobacterium tuberculosis strain 96121

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: HR37_RS14890
• Protein ID: WP_003414166.1
• Coordinates: 3119993..3120349 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: HR37_RS14895
• Protein ID: WP_003901465.1
• Coordinates: 3120333..3120563 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR37_RS14870	3115445..3117133	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
HR37_RS14875	3117137..3117463	-	327	WP_003414157.1	hypothetical protein	-
HR37_RS14880	3117636..3118223	+	588	WP_003914429.1	DUF3558 family protein	-
HR37_RS14885	3118242..3119891	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
HR37_RS14890	3119993..3120349	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
HR37_RS14895	3120333..3120563	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
HR37_RS14900	3120606..3121649	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
HR37_RS22295	3121840..3122115	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
HR37_RS22495	3122291..3122545	-	255	WP_003917684.1	hypothetical protein	-
HR37_RS14915	3122693..3123097	+	405	WP_003414181.1	hypothetical protein	-
HR37_RS14920	3123094..3123285	+	192	WP_003414184.1	hypothetical protein	-
HR37_RS14925	3123349..3124638	+	1290	Protein_2915	transposase family protein	-
HR37_RS14930	3124872..3125129	+	258	WP_003899489.1	hypothetical protein	-
HR37_RS14935	3125234..3125545	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3104637..3132031	27394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T48982 WP_003414166.1 NZ_CP009427:c3120349-3119993 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T48982 NZ_CP009427:c3120349-3119993 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT48982 WP_003901465.1 NZ_CP009427:c3120563-3120333 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT48982 NZ_CP009427:c3120563-3120333 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3