Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2879925..2880562 Replicon chromosome
Accession NZ_CP009427
Organism Mycobacterium tuberculosis strain 96121

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag HR37_RS13595 Protein ID WP_003413180.1
Coordinates 2879925..2880320 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag HR37_RS13600 Protein ID WP_003413183.1
Coordinates 2880317..2880562 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR37_RS13555 2875328..2876539 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
HR37_RS13560 2876666..2877325 + 660 WP_003900846.1 LppA family lipoprotein -
HR37_RS13565 2877322..2877984 + 663 WP_003903936.1 hypothetical protein -
HR37_RS13570 2877981..2878259 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
HR37_RS13575 2878352..2878765 + 414 WP_003413164.1 PIN domain nuclease -
HR37_RS13580 2878804..2879061 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
HR37_RS13585 2879058..2879435 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
HR37_RS13590 2879451..2879825 - 375 WP_003413177.1 hypothetical protein -
HR37_RS13595 2879925..2880320 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
HR37_RS13600 2880317..2880562 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
HR37_RS13605 2880973..2881392 - 420 WP_003413190.1 A24 family peptidase -
HR37_RS13610 2881404..2882213 - 810 WP_003413193.1 shikimate dehydrogenase -
HR37_RS13615 2882210..2883463 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
HR37_RS13620 2883456..2883968 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T48977 WP_003413180.1 NZ_CP009427:c2880320-2879925 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T48977 NZ_CP009427:c2880320-2879925 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT48977 WP_003413183.1 NZ_CP009427:c2880562-2880317 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT48977 NZ_CP009427:c2880562-2880317 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References