Detailed information of TA system TA048976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2878804..2879435
• Replicon: chromosome
• Accession: NZ_CP009427
• Organism: Mycobacterium tuberculosis strain 96121

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: HR37_RS13585
• Protein ID: WP_003413174.1
• Coordinates: 2879058..2879435 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: HR37_RS13580
• Protein ID: WP_003413167.1
• Coordinates: 2878804..2879061 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR37_RS13550	2874718..2875032	+	315	WP_009937839.1	hypothetical protein	-
HR37_RS13555	2875328..2876539	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
HR37_RS13560	2876666..2877325	+	660	WP_003900846.1	LppA family lipoprotein	-
HR37_RS13565	2877322..2877984	+	663	WP_003903936.1	hypothetical protein	-
HR37_RS13570	2877981..2878259	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
HR37_RS13575	2878352..2878765	+	414	WP_003413164.1	PIN domain nuclease	-
HR37_RS13580	2878804..2879061	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
HR37_RS13585	2879058..2879435	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
HR37_RS13590	2879451..2879825	-	375	WP_003413177.1	hypothetical protein	-
HR37_RS13595	2879925..2880320	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
HR37_RS13600	2880317..2880562	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
HR37_RS13605	2880973..2881392	-	420	WP_003413190.1	A24 family peptidase	-
HR37_RS13610	2881404..2882213	-	810	WP_003413193.1	shikimate dehydrogenase	-
HR37_RS13615	2882210..2883463	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
HR37_RS13620	2883456..2883968	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T48976 WP_003413174.1 NZ_CP009427:2879058-2879435 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T48976 NZ_CP009427:2879058-2879435 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT48976 WP_003413167.1 NZ_CP009427:2878804-2879061 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT48976 NZ_CP009427:2878804-2879061 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5