Detailed information of TA system TA048970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2339878..2340514
• Replicon: chromosome
• Accession: NZ_CP009427
• Organism: Mycobacterium tuberculosis strain 96121

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HR37_RS10980
• Protein ID: WP_003904769.1
• Coordinates: 2340104..2340514 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: HR37_RS10975
• Protein ID: WP_003410651.1
• Coordinates: 2339878..2340111 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR37_RS10965	2335728..2336132	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
HR37_RS10970	2336216..2339800	-	3585	WP_003410648.1	cobaltochelatase subunit CobN	-
HR37_RS10975	2339878..2340111	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
HR37_RS10980	2340104..2340514	+	411	WP_003904769.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
HR37_RS10985	2340498..2341589	+	1092	WP_003900454.1	precorrin-3B synthase	-
HR37_RS10990	2341599..2342225	+	627	WP_003410658.1	precorrin-8X methylmutase	-
HR37_RS10995	2342222..2343748	+	1527	WP_003410659.1	precorrin-2 C(20)-methyltransferase	-
HR37_RS11000	2343694..2344917	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14176.35 Da
• Isoelectric Point: 9.4835

>T48970 WP_003904769.1 NZ_CP009427:2340104-2340514 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMPAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T48970 NZ_CP009427:2340104-2340514 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCCCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT48970 WP_003410651.1 NZ_CP009427:2339878-2340111 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT48970 NZ_CP009427:2339878-2340111 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV50