Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2270733..2271359 Replicon chromosome
Accession NZ_CP009427
Organism Mycobacterium tuberculosis strain 96121

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag HR37_RS10675 Protein ID WP_003410075.1
Coordinates 2270961..2271359 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag HR37_RS10670 Protein ID WP_003911750.1
Coordinates 2270733..2270960 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR37_RS10660 2268772..2269116 - 345 WP_003410065.1 ferredoxin family protein -
HR37_RS10665 2269305..2270474 - 1170 WP_003899126.1 ATP-binding protein -
HR37_RS10670 2270733..2270960 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HR37_RS10675 2270961..2271359 + 399 WP_003410075.1 PIN domain nuclease Toxin
HR37_RS10680 2271542..2271973 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
HR37_RS10685 2272074..2272508 + 435 WP_003914358.1 DUF1398 domain-containing protein -
HR37_RS21425 2272945..2273124 - 180 Protein_2090 hypothetical protein -
HR37_RS10690 2273185..2273832 + 648 WP_071854215.1 transposase -
HR37_RS10695 2273786..2274376 + 591 WP_003907840.1 IS110 family transposase -
HR37_RS10700 2274504..2274878 - 375 Protein_2093 HNH endonuclease -
HR37_RS10710 2274929..2276190 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T48968 WP_003410075.1 NZ_CP009427:2270961-2271359 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T48968 NZ_CP009427:2270961-2271359 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT48968 WP_003911750.1 NZ_CP009427:2270733-2270960 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT48968 NZ_CP009427:2270733-2270960 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References