Detailed information of TA system TA048962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2202836..2203539
• Replicon: chromosome
• Accession: NZ_CP009427
• Organism: Mycobacterium tuberculosis strain 96121

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: HR37_RS10255
• Protein ID: WP_003409778.1
• Coordinates: 2202836..2203165 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: HR37_RS21395
• Protein ID: WP_003409780.1
• Coordinates: 2203162..2203539 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR37_RS10235	2199219..2200289	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
HR37_RS10240	2200286..2200801	+	516	WP_003409718.1	flavin reductase family protein	-
HR37_RS10245	2200798..2201859	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
HR37_RS10250	2201856..2202626	+	771	WP_003409775.1	SDR family oxidoreductase	-
HR37_RS10255	2202836..2203165	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HR37_RS21395	2203162..2203539	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
HR37_RS10265	2203536..2204126	-	591	WP_003409784.1	SEC-C domain-containing protein	-
HR37_RS10270	2204181..2205545	+	1365	WP_003903691.1	HNH endonuclease	-
HR37_RS10275	2205700..2206152	-	453	WP_003899095.1	lipoprotein	-
HR37_RS10280	2206216..2206617	+	402	WP_003409869.1	hypothetical protein	-
HR37_RS10285	2206610..2206792	-	183	WP_003409870.1	hypothetical protein	-
HR37_RS10290	2206906..2207256	-	351	WP_003409871.1	hypothetical protein	-
HR37_RS10295	2207267..2208169	-	903	WP_003409874.1	hypothetical protein	-
HR37_RS10300	2208190..2208381	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T48962 WP_003409778.1 NZ_CP009427:c2203165-2202836 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T48962 NZ_CP009427:c2203165-2202836 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT48962 WP_003409780.1 NZ_CP009427:c2203539-2203162 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT48962 NZ_CP009427:c2203539-2203162 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8