Detailed information of TA system TA048953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1234965..1235533
• Replicon: chromosome
• Accession: NZ_CP009427
• Organism: Mycobacterium tuberculosis strain 96121

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: HR37_RS05905
• Protein ID: WP_003405865.1
• Coordinates: 1235159..1235533 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: HR37_RS05900
• Protein ID: WP_003405863.1
• Coordinates: 1234965..1235162 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR37_RS05880	1231006..1231644	-	639	WP_003898730.1	lipid droplet-associated protein	-
HR37_RS05885	1231734..1232741	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
HR37_RS05890	1232758..1233741	-	984	WP_003903326.1	hypothetical protein	-
HR37_RS05895	1233804..1234877	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
HR37_RS05900	1234965..1235162	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HR37_RS05905	1235159..1235533	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
HR37_RS05910	1235736..1236434	+	699	WP_003898733.1	hypothetical protein	-
HR37_RS05915	1236552..1236737	+	186	WP_003901093.1	hypothetical protein	-
HR37_RS05920	1236664..1236939	-	276	WP_003405867.1	hypothetical protein	-
HR37_RS05925	1237182..1237505	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
HR37_RS05930	1237520..1238230	-	711	WP_003911399.1	hypothetical protein	-
HR37_RS22720	1238252..1238461	+	210	WP_003911400.1	hypothetical protein	-
HR37_RS05940	1238413..1239120	-	708	Protein_1160	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T48953 WP_003405865.1 NZ_CP009427:1235159-1235533 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T48953 NZ_CP009427:1235159-1235533 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT48953 WP_003405863.1 NZ_CP009427:1234965-1235162 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT48953 NZ_CP009427:1234965-1235162 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07