Detailed information of TA system TA048950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1021028..1022001
• Replicon: chromosome
• Accession: NZ_CP009427
• Organism: Mycobacterium tuberculosis strain 96121

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: HR37_RS04850
• Protein ID: WP_003404756.1
• Coordinates: 1021501..1022001 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: G0TFR4
• Locus tag: HR37_RS04845
• Protein ID: WP_003404754.1
• Coordinates: 1021028..1021504 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR37_RS04825	1016875..1018146	-	1272	WP_003404747.1	PPE family protein	-
HR37_RS04830	1018161..1018460	-	300	WP_003404748.1	PE family protein	-
HR37_RS04835	1018904..1020685	+	1782	WP_003903231.1	BCCT family transporter	-
HR37_RS04840	1020682..1020939	+	258	WP_003404750.1	hypothetical protein	-
HR37_RS04845	1021028..1021504	+	477	WP_003404754.1	DUF1778 domain-containing protein	Antitoxin
HR37_RS04850	1021501..1022001	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
HR37_RS04860	1022314..1023633	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
HR37_RS04865	1023921..1024502	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
HR37_RS04870	1024502..1026154	+	1653	WP_003903232.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T48950 WP_003404756.1 NZ_CP009427:1021501-1022001 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T48950 NZ_CP009427:1021501-1022001 [Mycobacterium tuberculosis]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT48950 WP_003404754.1 NZ_CP009427:1021028-1021504 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT48950 NZ_CP009427:1021028-1021504 [Mycobacterium tuberculosis]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTGGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2

Antitoxin

• AlphaFold DB: G0TFR4