Detailed information of TA system TA048949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1011498..1012117
• Replicon: chromosome
• Accession: NZ_CP009427
• Organism: Mycobacterium tuberculosis strain 96121

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: HR37_RS04795
• Protein ID: WP_003404726.1
• Coordinates: 1011683..1012117 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: HR37_RS04790
• Protein ID: WP_003404724.1
• Coordinates: 1011498..1011677 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR37_RS04780	1006971..1008551	+	1581	WP_003908598.1	serine hydrolase	-
HR37_RS04785	1008548..1010941	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
HR37_RS22680	1011214..1011372	+	159	WP_003404720.1	hypothetical protein	-
HR37_RS04790	1011498..1011677	+	180	WP_003404724.1	antitoxin	Antitoxin
HR37_RS04795	1011683..1012117	+	435	WP_003404726.1	SRPBCC family protein	Toxin
HR37_RS04800	1012215..1012988	+	774	WP_003404735.1	VOC family protein	-
HR37_RS04805	1013053..1013502	+	450	WP_003404738.1	hypothetical protein	-
HR37_RS04810	1013637..1013867	-	231	WP_003898642.1	hypothetical protein	-
HR37_RS04815	1014034..1015542	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
HR37_RS04820	1015544..1016782	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T48949 WP_003404726.1 NZ_CP009427:1011683-1012117 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T48949 NZ_CP009427:1011683-1012117 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT48949 WP_003404724.1 NZ_CP009427:1011498-1011677 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT48949 NZ_CP009427:1011498-1011677 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5