48919

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3047399..3048078 Replicon chromosome
Accession NZ_CP009426
Organism Mycobacterium tuberculosis strain 96075

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag LJ70_RS14515 Protein ID WP_003414059.1
Coordinates 3047399..3047815 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag LJ70_RS14520 Protein ID WP_003414061.1
Coordinates 3047812..3048078 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJ70_RS14495 3043451..3044353 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
LJ70_RS14500 3044422..3045174 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
LJ70_RS14505 3045418..3045693 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
LJ70_RS14510 3045690..3047312 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
LJ70_RS14515 3047399..3047815 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
LJ70_RS14520 3047812..3048078 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LJ70_RS14525 3048104..3048499 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
LJ70_RS14530 3048496..3048765 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
LJ70_RS14535 3048775..3049869 - 1095 WP_003414068.1 restriction endonuclease subunit S -
LJ70_RS14540 3049866..3050285 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
LJ70_RS14545 3050359..3050838 - 480 WP_003414073.1 dihydrofolate reductase -
LJ70_RS14550 3050909..3051709 - 801 WP_003911953.1 thymidylate synthase -
LJ70_RS14555 3051865..3052602 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T48919 WP_003414059.1 NZ_CP009426:c3047815-3047399 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T48919 NZ_CP009426:c3047815-3047399 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT48919 WP_003414061.1 NZ_CP009426:c3048078-3047812 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT48919 NZ_CP009426:c3048078-3047812 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References