Detailed information of TA system TA048915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2847250..2847881
• Replicon: chromosome
• Accession: NZ_CP009426
• Organism: Mycobacterium tuberculosis strain 96075

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: LJ70_RS13445
• Protein ID: WP_003413174.1
• Coordinates: 2847504..2847881 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: LJ70_RS13440
• Protein ID: WP_003413167.1
• Coordinates: 2847250..2847507 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJ70_RS13415	2842750..2843955	-	1206	WP_003413027.1	chorismate synthase	-
LJ70_RS13425	2844431..2845642	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
LJ70_RS13430	2845769..2846431	+	663	WP_031697764.1	hypothetical protein	-
LJ70_RS22220	2846428..2846705	+	278	Protein_2622	type II toxin-antitoxin system VapB family antitoxin	-
LJ70_RS13435	2846798..2847211	+	414	WP_003413164.1	PIN domain nuclease	-
LJ70_RS13440	2847250..2847507	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
LJ70_RS13445	2847504..2847881	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJ70_RS13450	2847897..2848271	-	375	WP_003413177.1	hypothetical protein	-
LJ70_RS13455	2848371..2848766	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
LJ70_RS13460	2848763..2849008	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
LJ70_RS13465	2849419..2849838	-	420	WP_003413190.1	A24 family peptidase	-
LJ70_RS13470	2849850..2850659	-	810	WP_003413193.1	shikimate dehydrogenase	-
LJ70_RS13475	2850656..2851909	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
LJ70_RS13480	2851902..2852414	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T48915 WP_003413174.1 NZ_CP009426:2847504-2847881 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T48915 NZ_CP009426:2847504-2847881 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT48915 WP_003413167.1 NZ_CP009426:2847250-2847507 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT48915 NZ_CP009426:2847250-2847507 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5