Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2787631..2788283 Replicon chromosome
Accession NZ_CP009426
Organism Mycobacterium tuberculosis strain 96075

Toxin (Protein)


Gene name vapC Uniprot ID A0A5R1ZCY8
Locus tag LJ70_RS13160 Protein ID WP_003905869.1
Coordinates 2787858..2788283 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag LJ70_RS13155 Protein ID WP_003412749.1
Coordinates 2787631..2787852 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJ70_RS13120 2783637..2783936 - 300 WP_003899346.1 hypothetical protein -
LJ70_RS13130 2784045..2785083 - 1039 Protein_2564 PE family protein -
LJ70_RS21510 2785232..2785387 + 156 Protein_2565 wax ester/triacylglycerol synthase family O-acyltransferase -
LJ70_RS13135 2785494..2785784 - 291 Protein_2566 PE family protein -
LJ70_RS13140 2785871..2786077 - 207 WP_003899347.1 hypothetical protein -
LJ70_RS13145 2786213..2786836 + 624 WP_003900858.1 TIGR00725 family protein -
LJ70_RS13150 2786826..2787578 + 753 WP_003901416.1 hypothetical protein -
LJ70_RS13155 2787631..2787852 + 222 WP_003412749.1 antitoxin Antitoxin
LJ70_RS13160 2787858..2788283 + 426 WP_003905869.1 type II toxin-antitoxin system VapC family toxin Toxin
LJ70_RS13165 2788306..2789487 - 1182 WP_003905870.1 2-oxo acid dehydrogenase subunit E2 -
LJ70_RS13170 2789484..2790530 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
LJ70_RS13175 2790541..2791644 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
LJ70_RS13180 2791903..2792724 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
LJ70_RS13185 2792721..2793278 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15364.84 Da        Isoelectric Point: 9.2386

>T48912 WP_003905869.1 NZ_CP009426:2787858-2788283 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRASTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T48912 NZ_CP009426:2787858-2788283 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT48912 WP_003412749.1 NZ_CP009426:2787631-2787852 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT48912 NZ_CP009426:2787631-2787852 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R1ZCY8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References