Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2175202..2175905 Replicon chromosome
Accession NZ_CP009426
Organism Mycobacterium tuberculosis strain 96075

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag LJ70_RS10115 Protein ID WP_003409778.1
Coordinates 2175202..2175531 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag LJ70_RS21305 Protein ID WP_003409780.1
Coordinates 2175528..2175905 (-) Length 126 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJ70_RS10095 2171585..2172655 + 1071 WP_003902252.1 epoxide hydrolase EphB -
LJ70_RS10100 2172652..2173167 + 516 WP_003409718.1 flavin reductase family protein -
LJ70_RS10105 2173164..2174225 + 1062 WP_003899092.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
LJ70_RS10110 2174222..2174992 + 771 WP_003409775.1 SDR family oxidoreductase -
LJ70_RS10115 2175202..2175531 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LJ70_RS21305 2175528..2175905 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
LJ70_RS10125 2175902..2176492 - 591 WP_003409784.1 SEC-C domain-containing protein -
LJ70_RS10130 2176547..2177911 + 1365 WP_003903691.1 HNH endonuclease -
LJ70_RS10135 2178066..2178518 - 453 WP_003899095.1 lipoprotein -
LJ70_RS10140 2178582..2178983 + 402 WP_003409869.1 hypothetical protein -
LJ70_RS10145 2178976..2179158 - 183 WP_003409870.1 hypothetical protein -
LJ70_RS10150 2179272..2179622 - 351 WP_003409871.1 hypothetical protein -
LJ70_RS10155 2179633..2180535 - 903 WP_003899097.1 hypothetical protein -
LJ70_RS10160 2180556..2180747 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-103)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T48901 WP_003409778.1 NZ_CP009426:c2175531-2175202 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T48901 NZ_CP009426:c2175531-2175202 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT48901 WP_003409780.1 NZ_CP009426:c2175905-2175528 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT48901 NZ_CP009426:c2175905-2175528 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TLK7


Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References