Detailed information of TA system TA048899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1937942..1938555
• Replicon: chromosome
• Accession: NZ_CP009426
• Organism: Mycobacterium tuberculosis strain 96075

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJ70_RS09030
• Protein ID: WP_038438632.1
• Coordinates: 1937942..1938331 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ52
• Locus tag: LJ70_RS09035
• Protein ID: WP_003408469.1
• Coordinates: 1938328..1938555 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJ70_RS08995	1933571..1934485	+	915	WP_003898985.1	3-hydroxyacyl-CoA dehydrogenase family protein	-
LJ70_RS09000	1934488..1935318	+	831	WP_003408448.1	cyclase family protein	-
LJ70_RS09005	1935318..1935668	+	351	WP_003898986.1	cupin domain-containing protein	-
LJ70_RS09010	1935721..1936539	+	819	WP_003408456.1	3-keto-5-aminohexanoate cleavage protein	-
LJ70_RS09015	1936553..1937332	+	780	WP_003408460.1	IclR family transcriptional regulator	-
LJ70_RS09030	1937942..1938331	-	390	WP_038438632.1	PIN domain-containing protein	Toxin
LJ70_RS09035	1938328..1938555	-	228	WP_003408469.1	antitoxin	Antitoxin
LJ70_RS09040	1938773..1940257	+	1485	WP_003408473.1	carboxylase	-
LJ70_RS09045	1940254..1941501	+	1248	WP_003408476.1	serine hydrolase	-
LJ70_RS09050	1941544..1941963	-	420	WP_003408483.1	hypothetical protein	-
LJ70_RS09055	1941953..1942663	-	711	WP_003408486.1	winged helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13832.00 Da
• Isoelectric Point: 7.0612

>T48899 WP_038438632.1 NZ_CP009426:c1938331-1937942 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTLADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Nucleotide

• Length: 390 bp

>T48899 NZ_CP009426:c1938331-1937942 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCTTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8226.39 Da
• Isoelectric Point: 4.3313

>AT48899 WP_003408469.1 NZ_CP009426:c1938555-1938328 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Nucleotide

• Length: 228 bp

>AT48899 NZ_CP009426:c1938555-1938328 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4FAN9