Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4749363..4750005 Replicon chromosome
Accession NZ_CP009369
Organism Bacillus cereus strain FM1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BG03_RS26220 Protein ID WP_000635963.1
Coordinates 4749363..4749713 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BG03_RS26225 Protein ID WP_000004570.1
Coordinates 4749718..4750005 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG03_RS26205 4746299..4746757 - 459 WP_001975527.1 SprT family protein -
BG03_RS26210 4746953..4747069 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BG03_RS26215 4747127..4749295 - 2169 WP_001975517.1 RNA-binding transcriptional accessory protein -
BG03_RS26220 4749363..4749713 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BG03_RS26225 4749718..4750005 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BG03_RS26230 4750315..4751484 - 1170 WP_000390607.1 alanine racemase -
BG03_RS26235 4751602..4752552 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BG03_RS26240 4752709..4753068 - 360 WP_000635047.1 holo-ACP synthase -
BG03_RS26245 4753161..4753733 + 573 WP_001975497.1 rhomboid family intramembrane serine protease -
BG03_RS26250 4753726..4754688 - 963 WP_042515799.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T48846 WP_000635963.1 NZ_CP009369:c4749713-4749363 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T48846 NZ_CP009369:c4749713-4749363 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT48846 WP_000004570.1 NZ_CP009369:c4750005-4749718 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT48846 NZ_CP009369:c4750005-4749718 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References