Detailed information of TA system TA048845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2580895..2581870
• Replicon: chromosome
• Accession: NZ_CP009369
• Organism: Bacillus cereus strain FM1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BG03_RS15160
• Protein ID: WP_033691453.1
• Coordinates: 2581133..2581870 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D8GY54
• Locus tag: BG03_RS15155
• Protein ID: WP_000594608.1
• Coordinates: 2580895..2581020 (-)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG03_RS15130	2575915..2576532	-	618	WP_142309569.1	SGNH/GDSL hydrolase family protein	-
BG03_RS15135	2576865..2577524	-	660	WP_042515123.1	CatB-related O-acetyltransferase	-
BG03_RS15140	2577979..2578449	+	471	WP_042515124.1	DUF5065 family protein	-
BG03_RS15145	2579030..2580499	-	1470	WP_001979053.1	beta-Ala-His dipeptidase	-
BG03_RS31235	2580643..2580819	-	177	WP_000852618.1	hypothetical protein	-
BG03_RS15155	2580895..2581020	-	126	WP_000594608.1	hypothetical protein	Antitoxin
BG03_RS15160	2581133..2581870	-	738	WP_033691453.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BG03_RS15165	2582059..2582796	-	738	WP_001980023.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BG03_RS15170	2582963..2583445	-	483	WP_000191902.1	MarR family winged helix-turn-helix transcriptional regulator	-
BG03_RS15175	2583552..2585237	+	1686	WP_052495880.1	alpha-keto acid decarboxylase family protein	-
BG03_RS15180	2585414..2586196	-	783	WP_042515126.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28433.19 Da
• Isoelectric Point: 8.2672

>T48845 WP_033691453.1 NZ_CP009369:c2581870-2581133 [Bacillus cereus]
VISNIRIGLFILVIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEVAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T48845 NZ_CP009369:c2581870-2581133 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGTAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCGAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACAGTTCAATCGATAAAACGTTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGTGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACTATGATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4878.27 Da
• Isoelectric Point: 3.9507

>AT48845 WP_000594608.1 NZ_CP009369:c2581020-2580895 [Bacillus cereus]
VIKNNDNDFFKEDNEDTTDFSLIKGDTMQQVEDLEDELKRK

Nucleotide

• Length: 126 bp

>AT48845 NZ_CP009369:c2581020-2580895 [Bacillus cereus]
GTGATAAAAAATAATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACGGATTTCTCGTTAATTAAAGGGGATAC
GATGCAGCAAGTAGAAGATTTAGAAGATGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U6H9M7