Detailed information of TA system TA048826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4229728..4230368
• Replicon: chromosome
• Accession: NZ_CP009365
• Organism: Pseudomonas soli strain SJ10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: O165_RS19485
• Protein ID: WP_023631112.1
• Coordinates: 4229958..4230368 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1I4A9
• Locus tag: O165_RS19480
• Protein ID: WP_011535889.1
• Coordinates: 4229728..4229958 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O165_RS19460	4225185..4226510	-	1326	WP_038706878.1	precorrin-3B synthase	-
O165_RS19465	4226622..4227833	+	1212	WP_038707606.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
O165_RS19470	4227826..4228920	+	1095	WP_038706879.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
O165_RS19475	4228917..4229645	+	729	WP_031315366.1	cobalt-precorrin-6A reductase	-
O165_RS19480	4229728..4229958	+	231	WP_011535889.1	antitoxin	Antitoxin
O165_RS19485	4229958..4230368	+	411	WP_023631112.1	tRNA(fMet)-specific endonuclease VapC	Toxin
O165_RS19490	4230441..4230878	+	438	WP_023631113.1	NfeD family protein	-
O165_RS19495	4230905..4231759	+	855	WP_023631114.1	SPFH/Band 7/PHB domain protein	-
O165_RS19500	4231871..4232260	+	390	WP_031315367.1	DUF2946 domain-containing protein	-
O165_RS19505	4232308..4232793	+	486	WP_023631116.1	copper chaperone PCu(A)C	-
O165_RS19510	4232814..4233239	+	426	WP_023631117.1	DUF2946 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15199.54 Da
• Isoelectric Point: 9.9050

>T48826 WP_023631112.1 NZ_CP009365:4229958-4230368 [Pseudomonas soli]
MLRYMLDTNICIFTIKNKPQTVREAFNRHHGQLAISTVTLMELIYGAEKSATPARNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVTQPR

Nucleotide

• Length: 411 bp

>T48826 NZ_CP009365:4229958-4230368 [Pseudomonas soli]
ATGCTCAGGTACATGCTCGACACCAACATCTGCATTTTCACCATCAAGAACAAACCACAAACGGTGCGCGAGGCGTTCAA
CCGCCATCATGGCCAACTGGCCATCAGCACGGTAACCCTGATGGAGTTGATCTACGGCGCGGAAAAGTCAGCCACGCCAG
CGCGTAATCTGGCCATTGTCGAGGGATTCGCCGCACGTCTCGATGTACTCGATTACGACATCCAGGGCGCTGCTCATACC
GCGCAACTGCGAGCGGAGCTGGCCAAGGCCGGTACCCCCATCGGCCCCTACGACCGAATGATCGCCGGCCACGCCCGCGC
CCGTGGCCTCACCCTGGTGACCAACAACCTACGCGAGTTCCAGCGTGTACCCGGGCTGCGTGTGGAGGACTGGGTGACAC
AACCACGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8443.36 Da
• Isoelectric Point: 4.2821

>AT48826 WP_011535889.1 NZ_CP009365:4229728-4229958 [Pseudomonas soli]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSADFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT48826 NZ_CP009365:4229728-4229958 [Pseudomonas soli]
ATGGAGCAAGGTGCAGTCTTCAAAAGCAACCGCAGCCAGGCTATCCGCATGCCAAAAGCCCTCGCCCTCCCAGAAGACGT
CACCCGCGTGGATATCGTCGCGGTCGGGCGTTCGCGCATCATCTCGCCCGCCGGCGAAAGCTGGGACAGCTGGTTCGAGG
GTGACGATGTGAGTGCGGACTTCATGGCTAGCAGGGAGCAACCGGCCGACCAGGAGCGCGAAGGATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2V4HYG6