Detailed information of TA system TA048819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2238679..2239297
• Replicon: chromosome
• Accession: NZ_CP009364
• Organism: Yersinia frederiksenii Y225

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: AW19_RS10565
• Protein ID: WP_002208622.1
• Coordinates: 2239094..2239297 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A386HG68
• Locus tag: AW19_RS10560
• Protein ID: WP_038631539.1
• Coordinates: 2238679..2239047 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AW19_RS10530	2234352..2234612	+	261	WP_038631547.1	type B 50S ribosomal protein L31	-
AW19_RS10535	2234628..2234771	+	144	WP_038631546.1	type B 50S ribosomal protein L36	-
AW19_RS10540	2234893..2235771	-	879	WP_038631545.1	metal ABC transporter substrate-binding protein	-
AW19_RS10545	2235818..2236675	-	858	WP_038631544.1	metal ABC transporter permease	-
AW19_RS10550	2236672..2237400	-	729	WP_038631543.1	ABC transporter ATP-binding protein	-
AW19_RS10555	2238167..2238520	+	354	WP_038631541.1	hypothetical protein	-
AW19_RS10560	2238679..2239047	+	369	WP_038631539.1	Hha toxicity modulator TomB	Antitoxin
AW19_RS10565	2239094..2239297	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
AW19_RS10570	2240254..2240565	+	312	WP_186368485.1	MGMT family protein	-
AW19_RS10575	2240609..2241133	-	525	WP_038631533.1	YbaY family lipoprotein	-
AW19_RS10580	2241391..2242251	+	861	WP_038631531.1	acyl-CoA thioesterase II	-
AW19_RS10585	2242333..2243622	-	1290	WP_038639390.1	ammonium transporter AmtB	-
AW19_RS10590	2243662..2244000	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T48819 WP_002208622.1 NZ_CP009364:2239094-2239297 [Yersinia frederiksenii Y225]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T48819 NZ_CP009364:2239094-2239297 [Yersinia frederiksenii Y225]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACAACTATTGATACATTAGAACGTGTAATTGAAAAGAATAA
GTACGAACTTTCAGATGATGAGCTGGAATTATTTTATTCAGCTGCAGACCATCGCTTAGCTGAGCTCACAATGAATAAAC
TTTATGATAAAATTCCACCTACTGTGTGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14190.97 Da
• Isoelectric Point: 4.5140

>AT48819 WP_038631539.1 NZ_CP009364:2238679-2239047 [Yersinia frederiksenii Y225]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDGADLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYICTLMKT

Nucleotide

• Length: 369 bp

>AT48819 NZ_CP009364:2238679-2239047 [Yersinia frederiksenii Y225]
ATGGATGAGTACTCACCGAAGCGGCATGACATTGCCCAATTGAAGTTTTTATGTGAGAGTTTGTATGATGAAGGCATTGC
CACCCTTGGTGACAGCCACCATGGTTGGGTAAATGACCCGACTTCTGCCGTAAATCTACAATTGAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCTGATGGCGCAGACCTTTCAGAGTTAGTGGAAGAATATTTA
GACGATACATATACTCTGTTTAGTAGTTATGGGATCAATGACCCTGAATTGCGTCGCTGGCAAAAAACCAAAGAGCGTTT
ATTCAGACTTTTCTCAGGGGAATACATCTGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S