Detailed information of TA system TA048785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 96464..97020
• Replicon: plasmid p123
• Accession: NZ_CP009357
• Organism: Vibrio tubiashii ATCC 19109

Toxin (Protein)

• Gene name: parE
• Uniprot ID: F9T1E7
• Locus tag: IX91_RS24765
• Protein ID: WP_004743243.1
• Coordinates: 96730..97020 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F9T1E6
• Locus tag: IX91_RS24760
• Protein ID: WP_004743242.1
• Coordinates: 96464..96742 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IX91_RS24735	93545..94189	-	645	WP_041944862.1	recombinase family protein	-
IX91_RS24740	94186..94461	-	276	WP_004743239.1	hypothetical protein	-
IX91_RS24750	95193..96008	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
IX91_RS24755	96095..96337	+	243	Protein_111	phosphoglucosamine mutase	-
IX91_RS24760	96464..96742	+	279	WP_004743242.1	CopG family ribbon-helix-helix protein	Antitoxin
IX91_RS24765	96730..97020	+	291	WP_004743243.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IX91_RS24775	97502..97828	+	327	WP_004743245.1	hypothetical protein	-
IX91_RS24780	97828..98475	+	648	WP_004743246.1	type IV secretion protein	-
IX91_RS24785	98485..98748	+	264	WP_004743247.1	EexN family lipoprotein	-
IX91_RS24790	98758..99756	+	999	WP_004743248.1	type IV secretion system protein	-
IX91_RS24800	100333..101169	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
IX91_RS24805	101169..101972	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(6)-Id / aph(3'')-Ib	-	1..122808	122808
	-	inside	IScluster/Tn	sul2 / aph(6)-Id / aph(3'')-Ib	-	90463..105069	14606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10822.41 Da
• Isoelectric Point: 5.1532

>T48785 WP_004743243.1 NZ_CP009357:96730-97020 [Vibrio tubiashii ATCC 19109]
MSQVIFAPAAIQDLERLREFLRPKNPTAAERAAKAIIQGVQALGELPRIGRPIEDMPEEFRDWLIDFGDSGYVARYRIDG
DTVVVLAIRHQKEAGF

Nucleotide

• Length: 291 bp

>T48785 NZ_CP009357:96730-97020 [Vibrio tubiashii ATCC 19109]
ATGTCACAAGTGATCTTCGCCCCGGCAGCGATCCAGGACCTGGAGCGGCTGCGCGAGTTTCTACGGCCCAAGAACCCCAC
AGCAGCGGAGCGGGCCGCTAAAGCCATCATCCAGGGTGTCCAGGCGCTCGGAGAACTGCCGCGCATTGGCCGCCCCATTG
AGGATATGCCCGAGGAGTTCCGGGACTGGCTGATAGACTTTGGTGATAGCGGCTATGTCGCACGATACCGTATTGATGGT
GACACCGTTGTGGTGTTGGCCATCCGTCACCAGAAAGAAGCCGGCTTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10750.97 Da
• Isoelectric Point: 6.4837

>AT48785 WP_004743242.1 NZ_CP009357:96464-96742 [Vibrio tubiashii ATCC 19109]
MATPTSIKLDDDLKGRVQHLADTRRRSAHWLMREAIEQYVEREERRESFRRDTLKAWEDYQATGLHATAGEVEKWLASWG
TDNEVPAPTCHK

Nucleotide

• Length: 279 bp

>AT48785 NZ_CP009357:96464-96742 [Vibrio tubiashii ATCC 19109]
ATGGCAACACCAACATCCATCAAGCTCGATGACGATCTGAAAGGGCGGGTCCAGCATCTGGCCGATACTCGTAGGCGCTC
GGCGCACTGGCTCATGCGTGAAGCAATCGAACAGTACGTGGAGCGCGAGGAAAGGCGGGAGAGCTTCAGAAGAGACACGC
TCAAGGCTTGGGAGGACTACCAGGCTACCGGCTTGCACGCGACCGCTGGCGAAGTGGAGAAGTGGCTGGCCAGTTGGGGA
ACGGATAACGAGGTGCCGGCCCCGACATGTCACAAGTGA

Structures

Toxin

• AlphaFold DB: F9T1E7

Antitoxin

• AlphaFold DB: F9T1E6