Detailed information of TA system TA048782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 1563056..1564034
• Replicon: chromosome
• Accession: NZ_CP009351
• Organism: Bacillus thuringiensis HD1002

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: AS86_RS13540
• Protein ID: WP_000624969.1
• Coordinates: 1563297..1564034 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B7IX39
• Locus tag: AS86_RS13535
• Protein ID: WP_000237817.1
• Coordinates: 1563056..1563184 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS86_RS13505	1558573..1558824	+	252	WP_000821942.1	response regulator transcription factor	-
AS86_RS13510	1558965..1559570	-	606	WP_000527414.1	hypothetical protein	-
AS86_RS13515	1559596..1560405	-	810	WP_000678376.1	C39 family peptidase	-
AS86_RS13520	1560718..1562187	-	1470	WP_000287541.1	beta-Ala-His dipeptidase	-
AS86_RS13525	1562399..1562788	+	390	WP_000712655.1	YxeA family protein	-
AS86_RS13530	1562807..1562983	-	177	WP_000852629.1	stage II sporulation protein SB	-
AS86_RS13535	1563056..1563184	-	129	WP_000237817.1	hypothetical protein	Antitoxin
AS86_RS13540	1563297..1564034	-	738	WP_000624969.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
AS86_RS13545	1564273..1565010	-	738	WP_000594159.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
AS86_RS13550	1565176..1565658	-	483	WP_000191905.1	MarR family winged helix-turn-helix transcriptional regulator	-
AS86_RS35125	1565766..1565934	+	169	Protein_1567	hypothetical protein	-
AS86_RS13555	1565910..1566755	+	846	Protein_1568	alpha-keto acid decarboxylase family protein	-
AS86_RS13560	1566909..1567691	-	783	WP_000381785.1	class I SAM-dependent methyltransferase	-
AS86_RS13565	1568036..1568443	-	408	WP_000072495.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (11-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28383.13 Da
• Isoelectric Point: 8.2998

>T48782 WP_000624969.1 NZ_CP009351:c1564034-1563297 [Bacillus thuringiensis HD1002]
MISNIRIGLFVLAIFFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T48782 NZ_CP009351:c1564034-1563297 [Bacillus thuringiensis HD1002]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTTTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACCACAATTATTCAAAATGTTAATCCAGCAGTTTTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAGAGCTTAAATGTATTTTTAGATTCATATGGGGAAAAAATTGGCGCGAAAATT
GTTGTTTTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4981.37 Da
• Isoelectric Point: 3.9150

>AT48782 WP_000237817.1 NZ_CP009351:c1563184-1563056 [Bacillus thuringiensis HD1002]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT48782 NZ_CP009351:c1563184-1563056 [Bacillus thuringiensis HD1002]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATGC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: B7IX39