Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4263358..4264000 Replicon chromosome
Accession NZ_CP009328
Organism Bacillus anthracis strain Vollum 1B

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BF39_RS24220 Protein ID WP_000635963.1
Coordinates 4263650..4264000 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BF39_RS24215 Protein ID WP_000004570.1
Coordinates 4263358..4263645 (+) Length 96 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BF39_RS24190 4258674..4259636 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
BF39_RS24195 4259629..4260201 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
BF39_RS24200 4260294..4260653 + 360 WP_000635040.1 holo-ACP synthase -
BF39_RS24205 4260810..4261760 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BF39_RS24210 4261879..4263048 + 1170 WP_000390596.1 alanine racemase -
BF39_RS24215 4263358..4263645 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BF39_RS24220 4263650..4264000 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BF39_RS24225 4264068..4266236 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BF39_RS24230 4266294..4266410 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BF39_RS24235 4266606..4267064 + 459 WP_000344248.1 SprT family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-113)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T48772 WP_000635963.1 NZ_CP009328:4263650-4264000 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T48772 NZ_CP009328:4263650-4264000 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT48772 WP_000004570.1 NZ_CP009328:4263358-4263645 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT48772 NZ_CP009328:4263358-4263645 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY


Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References