Detailed information of TA system TA048769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2894937..2895912
• Replicon: chromosome
• Accession: NZ_CP009325
• Organism: Bacillus anthracis strain PAK-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A2R2IKP2
• Locus tag: BD64_RS16375
• Protein ID: WP_002036394.1
• Coordinates: 2895175..2895912 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: BD64_RS16370
• Protein ID: WP_000588712.1
• Coordinates: 2894937..2895062 (-)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BD64_RS16340	2890691..2891161	+	471	WP_000670597.1	DUF5065 family protein	-
BD64_RS16345	2891725..2893194	-	1470	WP_000287522.1	beta-Ala-His dipeptidase	-
BD64_RS31525	2893338..2893514	-	177	WP_000808045.1	hypothetical protein	-
BD64_RS16355	2893853..2894089	+	237	WP_001021689.1	hypothetical protein	-
BD64_RS16360	2894366..2894647	+	282	WP_001041555.1	hypothetical protein	-
BD64_RS31530	2894684..2894860	-	177	WP_000852614.1	hypothetical protein	-
BD64_RS16370	2894937..2895062	-	126	WP_000588712.1	hypothetical protein	Antitoxin
BD64_RS16375	2895175..2895912	-	738	WP_002036394.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BD64_RS16380	2896101..2896838	-	738	WP_000594139.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BD64_RS16385	2897006..2897488	-	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
BD64_RS16390	2897595..2899280	+	1686	WP_042513802.1	alpha-keto acid decarboxylase family protein	-
BD64_RS16395	2899402..2900184	-	783	WP_000381787.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.03 Da
• Isoelectric Point: 8.2705

>T48769 WP_002036394.1 NZ_CP009325:c2895912-2895175 [Bacillus anthracis]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T48769 NZ_CP009325:c2895912-2895175 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT48769 WP_000588712.1 NZ_CP009325:c2895062-2894937 [Bacillus anthracis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT48769 NZ_CP009325:c2895062-2894937 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2R2IKP2

Antitoxin

• AlphaFold DB: A0A366GQT1