Detailed information of TA system TA048762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 15892..16445
• Replicon: plasmid 3
• Accession: NZ_CP009319
• Organism: Burkholderia gladioli strain ATCC 10248

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BM43_RS37905
• Protein ID: WP_080742290.1
• Coordinates: 16152..16445 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A808W488
• Locus tag: BM43_RS00110
• Protein ID: WP_042286915.1
• Coordinates: 15892..16164 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BM43_RS00085	11612..12145	-	534	WP_042286924.1	PAAR domain-containing protein	-
BM43_RS00090	12182..12841	-	660	WP_052409305.1	hypothetical protein	-
BM43_RS00095	12960..13262	-	303	WP_042286921.1	hypothetical protein	-
BM43_RS39900	13259..13759	-	501	WP_144417590.1	hypothetical protein	-
BM43_RS00100	13834..14142	+	309	WP_042286918.1	MobB mobilization protein	-
BM43_RS00105	14139..15812	+	1674	WP_063769171.1	relaxase/mobilization nuclease domain-containing protein	-
BM43_RS00110	15892..16164	+	273	WP_042286915.1	CopG family ribbon-helix-helix protein	Antitoxin
BM43_RS37905	16152..16445	+	294	WP_080742290.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BM43_RS00115	16442..16762	+	321	WP_042286911.1	hypothetical protein	-
BM43_RS00120	17035..17214	+	180	WP_042286906.1	hypothetical protein	-
BM43_RS00125	17204..17518	+	315	WP_042286903.1	hypothetical protein	-
BM43_RS00130	17552..18061	-	510	WP_042286898.1	hypothetical protein	-
BM43_RS00135	18073..20244	-	2172	WP_042286894.1	DotA/TraY family protein	-
BM43_RS00140	20241..20849	-	609	WP_042286891.1	hypothetical protein	-
BM43_RS41175	20807..21091	-	285	WP_146775969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..26710	26710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11000.51 Da
• Isoelectric Point: 8.0198

>T48762 WP_080742290.1 NZ_CP009319:16152-16445 [Burkholderia gladioli]
VPHVIITPRAGQGLERCRRFLADKSETAVRRAAQAIEQRFLLLETTPAVGRPFPENPAWRELAIPFGDSGYVALYRYEPA
DDAVYVLAFRHQREAGY

Nucleotide

• Length: 294 bp

>T48762 NZ_CP009319:16152-16445 [Burkholderia gladioli]
GTGCCACACGTAATTATCACGCCTCGCGCCGGTCAAGGATTGGAGCGCTGCCGGCGCTTTCTTGCCGACAAAAGCGAGAC
AGCCGTTCGGCGGGCGGCGCAGGCCATCGAGCAGCGATTTCTATTGCTGGAAACGACGCCGGCCGTTGGCCGCCCTTTTC
CTGAAAATCCGGCGTGGCGCGAGCTGGCCATTCCGTTCGGCGACTCTGGCTATGTCGCCTTGTATCGGTACGAACCGGCA
GATGATGCCGTCTACGTGCTTGCTTTCCGGCATCAGAGAGAGGCGGGGTATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10456.58 Da
• Isoelectric Point: 5.1678

>AT48762 WP_042286915.1 NZ_CP009319:15892-16164 [Burkholderia gladioli]
MATSLKIDDELKGRVQHLASTRDRSPHWIMCEAIRQYVEREEARESFKQEAAASWAHYQETGLHLTGEEVRTWLKTWGTD
DETGLPECHT

Nucleotide

• Length: 273 bp

>AT48762 NZ_CP009319:15892-16164 [Burkholderia gladioli]
ATGGCAACGTCACTCAAGATCGACGACGAGCTGAAGGGGCGCGTACAGCACCTTGCCAGCACGCGTGATCGGTCGCCGCA
CTGGATTATGTGCGAGGCAATCAGGCAGTATGTCGAGCGCGAAGAAGCGCGCGAAAGCTTCAAGCAGGAGGCTGCGGCCT
CCTGGGCTCACTATCAGGAAACCGGCCTGCATCTGACCGGGGAAGAGGTTCGCACCTGGCTGAAAACGTGGGGCACGGAC
GATGAAACGGGGCTGCCTGAGTGCCACACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808W488