Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2047021..2047663 Replicon chromosome
Accession NZ_CP009315
Organism Bacillus anthracis str. Turkey32 strain 35

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag AS53_RS11765 Protein ID WP_000635963.1
Coordinates 2047313..2047663 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag AS53_RS11760 Protein ID WP_000004570.1
Coordinates 2047021..2047308 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AS53_RS11735 2042337..2043299 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
AS53_RS11740 2043292..2043864 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
AS53_RS11745 2043957..2044316 + 360 WP_000635040.1 holo-ACP synthase -
AS53_RS11750 2044473..2045423 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
AS53_RS11755 2045542..2046711 + 1170 WP_000390596.1 alanine racemase -
AS53_RS11760 2047021..2047308 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
AS53_RS11765 2047313..2047663 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AS53_RS11770 2047731..2049899 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
AS53_RS11775 2049957..2050073 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
AS53_RS11780 2050269..2050727 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T48758 WP_000635963.1 NZ_CP009315:2047313-2047663 [Bacillus anthracis str. Turkey32]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T48758 NZ_CP009315:2047313-2047663 [Bacillus anthracis str. Turkey32]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT48758 WP_000004570.1 NZ_CP009315:2047021-2047308 [Bacillus anthracis str. Turkey32]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT48758 NZ_CP009315:2047021-2047308 [Bacillus anthracis str. Turkey32]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References