48757

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4277267..4277909 Replicon chromosome
Accession NZ_CP009300
Organism Bacillus cereus D17

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag AW22_RS23415 Protein ID WP_000635963.1
Coordinates 4277267..4277617 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag AW22_RS23420 Protein ID WP_000004570.1
Coordinates 4277622..4277909 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW22_RS23400 4274203..4274661 - 459 WP_000344244.1 SprT family protein -
AW22_RS23405 4274857..4274973 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
AW22_RS23410 4275031..4277199 - 2169 WP_042513310.1 RNA-binding transcriptional accessory protein -
AW22_RS23415 4277267..4277617 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AW22_RS23420 4277622..4277909 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
AW22_RS23425 4278219..4279388 - 1170 WP_000390600.1 alanine racemase -
AW22_RS23430 4279507..4280457 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
AW22_RS23435 4280614..4280973 - 360 WP_000635040.1 holo-ACP synthase -
AW22_RS23440 4281066..4281638 + 573 WP_000906926.1 rhomboid family intramembrane serine protease -
AW22_RS23445 4281631..4282593 - 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T48757 WP_000635963.1 NZ_CP009300:c4277617-4277267 [Bacillus cereus D17]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T48757 NZ_CP009300:c4277617-4277267 [Bacillus cereus D17]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT48757 WP_000004570.1 NZ_CP009300:c4277909-4277622 [Bacillus cereus D17]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT48757 NZ_CP009300:c4277909-4277622 [Bacillus cereus D17]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References