Detailed information of TA system TA048753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 608047..608584
• Replicon: chromosome
• Accession: NZ_CP009297
• Organism: Burkholderia pseudomallei 406e

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A0F6L9W9
• Locus tag: BG92_RS02550
• Protein ID: WP_009971435.1
• Coordinates: 608047..608316 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BG92_RS02555
• Protein ID: WP_004525420.1
• Coordinates: 608300..608584 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG92_RS40875	604043..604471	-	429	Protein_501	DDE-type integrase/transposase/recombinase	-
BG92_RS40880	604469..605242	-	774	Protein_502	prohibitin family protein	-
BG92_RS02515	605239..605376	-	138	WP_009971437.1	hypothetical protein	-
BG92_RS02520	605644..606225	+	582	WP_004542548.1	hypothetical protein	-
BG92_RS40230	606456..606755	-	300	WP_160469016.1	hypothetical protein	-
BG92_RS02530	606722..606943	-	222	WP_004538072.1	hypothetical protein	-
BG92_RS02545	607220..607495	-	276	WP_004537565.1	bacteriophage protein Gp49	-
BG92_RS02550	608047..608316	+	270	WP_009971435.1	BrnT family toxin	Toxin
BG92_RS02555	608300..608584	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BG92_RS02565	609231..609614	+	384	Protein_510	DDE-type integrase/transposase/recombinase	-
BG92_RS31955	609577..610005	+	429	Protein_511	IS110 family transposase	-
BG92_RS02570	610232..610348	-	117	Protein_512	IS3 family transposase	-
BG92_RS02575	610595..612055	+	1461	WP_004538070.1	metallophosphoesterase	-
BG92_RS02580	612214..613476	+	1263	WP_004537187.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	601732..612055	10323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10143.43 Da
• Isoelectric Point: 5.6831

>T48753 WP_009971435.1 NZ_CP009297:608047-608316 [Burkholderia pseudomallei 406e]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVVGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T48753 NZ_CP009297:608047-608316 [Burkholderia pseudomallei 406e]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGGTCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT48753 WP_004525420.1 NZ_CP009297:608300-608584 [Burkholderia pseudomallei 406e]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT48753 NZ_CP009297:608300-608584 [Burkholderia pseudomallei 406e]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0F6L9W9

Antitoxin

• AlphaFold DB: A0A0F6L9S8